The Hpk2-Rrp2 two-component regulatory system of Treponema denticola: a potential regulator of environmental and adaptive responses.
The Hpk2-Rrp2 two-component regulatory system of Treponema denticola: a potential regulator of environmental and adaptive responses.
复制标题
齿垢密螺旋体的 Hpk2-Rrp2 双组分调节系统：环境和适应性反应的潜在调节剂。
DOI：
10.1111/j.2041-1014.2010.00578.x
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发表时间：
2010
影响因子：
3.7
通讯作者：
Marconi,RT
中科院分区：
文献类型：
--
作者：
Sarkar,J;Frederick,J;Marconi,RT
随着牙周病的发展，牙龈缝隙中的密螺旋体水平升高。口腔密螺旋体可能占牙周袋内细菌总数的40%。刺激经济增长的因素。Denticola以及与这些信号传递相关的机制仍有待确定。我们假设。Denticolaopen阅读框架de1970（组氨酸激酶）和tde1969（反应调节因子）构成了一个功能性的双组分调节系统，至少部分地调节对与牙周病发展相关的环境条件变化的反应。结果表明，1970和1969是保守的，具有普适性。在生长阶段依赖的方式下，作为7个基因操纵子的一部分进行转录。Tde1970经历自磷酸化并将磷酸盐转移到tde1969。因此，这些开放阅读框编码的蛋白分别被命名为Hpk2和Rrp2。Hpk2的自磷酸化动力学受到环境条件和PAS结构域存在与否的影响。由此可见，Hpk2和Rrp2构成了一个具有环境感知功能的双组分系统。
Treponema denticolalevels in the gingival crevice become elevated as periodontal disease develops. Oral treponemes may account for as much as 40% of the total bacterial population in the periodontal pocket. The stimuli that trigger enhanced growth ofT. denticola, and the mechanisms associated with the transmission of these signals, remain to be defined. We hypothesize that theT. denticolaopen reading framestde1970(histidine kinase) andtde1969(response regulator) constitute a functional two‐component regulatory system that regulates, at least in part, responses to the changing environmental conditions associated with the development of periodontal disease. The results presented demonstrate thattde1970andtde1969are conserved, universal amongT. denticolaisolates and transcribed as part of a seven‐gene operon in a growth‐phase‐dependent manner. tde1970 undergoes autophosphorylation and transfers phosphate to tde1969. Henceforth, the proteins encoded by these open reading frames are designated as Hpk2 and Rrp2 respectively. Hpk2 autophosphorylation kinetics were influenced by environmental conditions and by the presence or absence of a PAS domain. It can be concluded that Hpk2 and Rrp2 constitute a functional two‐component system that contributes to environmental sensing.