An apparatus and behavioral training protocol to conduct positron emission tomography (PET) neuroimaging in conscious rhesus monkeys
An apparatus and behavioral training protocol to conduct positron emission tomography (PET) neuroimaging in conscious rhesus monkeys
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DOI：
10.1016/s0165-0270(01)00345-4
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发表时间：
2001-04-30
影响因子：
3
通讯作者：
Jordan, JF
中科院分区：
文献类型：
--
作者：
Howell, LL;Hoffman, JM;Jordan, JF
非人灵长类动物在神经成像研究中提供了独特的资源，提供了在良好控制的实验条件下，在与人类在功能和神经解剖学上与人类密切相关的动物模型中操纵适当的生物和行为变量的机会。本报告描述了清醒恒河猴的PET神经成像方案的发展和标准化，以及它们在表征可卡因对脑血流的急性影响方面的应用。特别关注的是开发一种有效和舒适的头部约束装置，用于清醒猴子的成像。这种约束装置被设计成固定在标准的灵长类座椅上，以便于频繁地固定。受试者在神经成像前接受了广泛的行为训练，以确保他们的舒适性，并将与成像方案相关的潜在压力降至最低。用O-15水正电子示踪剂对三名受试者急性静脉注射后脑血流的功能变化进行了研究。可卡因的使用。在MRI扫描上以高精度确定感兴趣区域。可卡因在注射后5min引起脑血流量显著增加，但在25min内明显减弱。这些结果证明了在有意识的非人类灵长类动物中进行脑血流PET神经成像研究的可行性。将方法论扩展到包括行为研究期间的大脑激活，可能会对行为神经科学的不断发展做出重大贡献。(C)2001 Elsevier Science B.V.保留所有权利。
Nonhuman primates offer a unique resource in neuroimaging research, providing the opportunity to manipulate appropriate biological and behavioral variables under well-controlled experimental conditions in an animal model that is closely related to humans, both functionally and neuroanatomically. The present report describes the development and standardization of PET neuroimaging protocols in conscious rhesus monkeys and their application to characterize the acute effects of cocaine on cerebral blood flow. Specific attention was devoted to the development of an effective and comfortable head restraint device to be used in the imaging of conscious monkeys. The restraint device was designed to attach to a standard primate chair to facilitate frequent immobilization. Subjects received extensive behavioral training prior to neuroimaging in order to ensure their comfort and minimize potential stress associated with the imaging protocols. Functional changes in cerebral blood flow were characterized in three subjects with the positron-emitting tracer O-15 water following acute i.v. administration of cocaine. Regions of interest were defined on MRI scans with a high degree of accuracy. Cocaine caused pronounced increases in cerebral blood flow at 5 min postinjection that diminished markedly within 25 min. The results document the feasibility to conduct PET neuroimaging studies of cerebral blood flow in conscious nonhuman primates. Extension of the methodology to include brain activation during behavioral studies could contribute significantly to the growing discipline of behavioral neuroscience. (C) 2001 Elsevier Science B.V. All rights reserved.