Two-photon imaging to a depth of 1000 μm in living brains by use of a Ti:Al2O3 regenerative amplifier
Two-photon imaging to a depth of 1000 μm in living brains by use of a Ti:Al2O3 regenerative amplifier
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DOI：
10.1364/ol.28.001022
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发表时间：
2003-06-15
期刊：
影响因子：
3.6
通讯作者：
Denk, W
中科院分区：
文献类型：
--
作者：
Theer, P;Hasan, MT;Denk, W
结果表明，通过使用光学放大的飞秒脉冲，可以在小鼠新皮质的几乎整个灰质中获得双光子荧光图像。所实现的成像深度接近由离焦荧光激发设定的理论极限。 (C) 2003 年美国光学学会。
It is shown that two-photon fluorescence images can be obtained throughout almost the entire gray matter of the mouse neocortex by using optically amplified femtosecond pulses. The achieved imaging depth approaches the theoretical limit set by excitation of out-of-focus fluorescence. (C) 2003 Optical Society of America.