Astrogliosis and blood vessel development during human spinal cord myelination.
Astrogliosis and blood vessel development during human spinal cord myelination.
复制标题
人脊髓髓鞘形成过程中的星形胶质细胞增生和血管发育。
作者：
D. Dziewulska;J. Rafałowska
本研究的目的是探讨人类血管发育、星形胶质细胞增生和髓鞘形成之间的关系。我们用抗髓鞘碱性蛋白（MBP）、胶质纤维酸性蛋白（GFAP）和凝集素荆豆1型（UEA-1）的免疫组织化学方法检查了人胎儿和婴儿的脊髓。我们的研究表明，在脊髓白色物质中MBP反应性增加的同时，出现了更多的GFAP免疫反应性星形胶质细胞。血管内皮细胞与凝集素UEA-1的反应揭示了胎儿脊髓束髓鞘形成过程中血管化的时间增加。在出生后的时期，当髓鞘形成几乎完成的形态，脊髓白色物质的血管化较差，虽然GFAP免疫反应细胞仍然相对较多。我们认为，在脊髓束髓鞘形成过程中观察到的血管化和星形胶质细胞增生的增加可能与它们通过正常髓鞘发育所需的生长因子的局部分泌参与髓鞘形成过程有关。
The aim of our study was to investigate a relationship between vessel development, astrogliosis and myelination in humans. We examined spinal cords of human fetuses and infants using immunohistochemical methods with antibodies against Myelin Basic Protein (MBP), Glial Fibrillary Acidic Protein (GFAP) and lectin Ulex Europaeus Type 1 (UEA-1). Our investigation showed that in parallel to the increase of MBP reactivity in the spinal cord white matter more GFAP-immunoreactive astrocytes appeared. Reaction of vessel endothelium with lectin UEA-1 revealed the temporal increase of vascularization in the course of the spinal cord tracts myelination in fetuses. In the postnatal period when the myelination is nearly morphologically completed, the spinal cord white matter was poorly vascularized although GFAP-immunoreactive cells were still relatively numerous. We suggested that the increase of vascularization and astrogliosis observed during spinal cord tracts myelination may be connected with their participation in process of myelin sheath formation via, among others, local secretion of growth factors necessary for normal myelin development.