Function of Rhodopsin in Temperature Discrimination in Drosophila
Function of Rhodopsin in Temperature Discrimination in Drosophila
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DOI：
10.1126/science.1198904
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发表时间：
2011-03-11
期刊：
影响因子：
56.9
通讯作者：
Montell, Craig
中科院分区：
文献类型：
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作者：
Shen, Wei L.;Kwon, Young;Montell, Craig
许多动物，包括果蝇，对环境温度的微小差异都很敏感。果蝇幼虫选择理想温度(18摄氏度)而不是其他舒适温度(19摄氏度至24摄氏度)的能力取决于一条温度敏感信号通路，该通路包括异源三聚体鸟嘌呤核苷酸结合蛋白(G蛋白)、磷脂酶C和瞬时受体电位TRPA1通道。我们报告了编码经典G蛋白偶联受体(GPCR)的基因(NINAE)的突变，果蝇视紫红质在舒适的温度范围内消除了趋热歧视。视紫红质在向18摄氏度趋热中的这种作用是不依赖于光的。小鼠黑素的引入恢复了突变幼虫的正常趋热行为。我们认为，视紫红质代表了一类进化上保守的GPCR，它们是启动热敏信号级联所必需的。
Many animals, including the fruit fly, are sensitive to small differences in ambient temperature. The ability of Drosophila larvae to choose their ideal temperature (18 degrees C) over other comfortable temperatures (19 degrees to 24 degrees C) depends on a thermosensory signaling pathway that includes a heterotrimeric guanine nucleotide-binding protein (G protein), a phospholipase C, and the transient receptor potential TRPA1 channel. We report that mutation of the gene (ninaE) encoding a classical G protein-coupled receptor (GPCR), Drosophila rhodopsin, eliminates thermotactic discrimination in the comfortable temperature range. This role for rhodopsin in thermotaxis toward 18 degrees C was light-independent. Introduction of mouse melanopsin restored normal thermotactic behavior in ninaE mutant larvae. We propose that rhodopsins represent a class of evolutionarily conserved GPCRs that are required for initiating thermosensory signaling cascades.