A device for the simple and rapid transcervical transfer of mouse embryos eliminates the need for surgery and potential post-operative complications.
A device for the simple and rapid transcervical transfer of mouse embryos eliminates the need for surgery and potential post-operative complications.
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DOI：
10.2144/000113257
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发表时间：
2009-11
期刊：
影响因子：
2.7
通讯作者：
Spear B
中科院分区：
文献类型：
--
作者：
Green M;Bass S;Spear B
我们描述了一种新型装置，可用于将胚胎经宫颈移植到假孕雌性小鼠中。这种非手术胚胎移植（NSET）装置在转基因小鼠的生产中与标准外科胚胎移植一样有效，并且还可用于含有胚胎干细胞的嵌合囊胚和冷冻胚胎的移植。除了消除手术外，受者女性也不必麻醉。 NSET 设备消除了痛苦的手术过程以及与麻醉/术后护理相关的潜在并发症，减少了手术转移所需的技术专业知识和设备，并节省了大量成本并减少了监管。 NSET 技术为外科胚胎移植提供了一种简单、快速的替代方案。
We describe a novel device that can be used for the transcervical transfer of embryos into pseudopregnant female mice. This nonsurgical embryo transfer (NSET) device is as efficient as standard surgical embryo transfer in the production of transgenic mice, and can also be used for the transfer of embryonic stem cell–containing chimeric blastocysts and cryopreserved embryos. In addition to the elimination of surgery, recipient females do not have to be anesthetized. The NSET device eliminates a painful surgical procedure as well as potential complications associated with anesthesia/post-operative care, reduces the technical expertise and equipment needed for surgical transfer, and represents substantial cost savings and regulatory reduction. NSET technology provides an easy and rapid alternative to surgical embryo transfer.