The pre-immune variable kappa repertoire of swine is selectively generated from certain subfamilies of Vκ2 and one Jκ gene
The pre-immune variable kappa repertoire of swine is selectively generated from certain subfamilies of Vκ2 and one Jκ gene
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DOI：
10.1016/j.vetimm.2005.07.016
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发表时间：
2005-10-18
影响因子：
1.8
通讯作者：
Wells, K
中科院分区：
文献类型：
--
作者：
Butler, JE;Wertz, N;Wells, K
组合多样性在猪的免疫前重链库的形成过程中受到高度限制，提出了免疫前轻链库是否也是如此的问题。在解决这个问题之前，我们首先使用竞争性PCR来显示猪的κ和λ轻链在成熟的B细胞中同样表达，这与人类的情况相似，但与其他研究的有蹄类动物相似。这证明了检查两种轻链类型库的努力是合理的。这些研究还揭示了λ优先在B细胞淋巴发生的位点表达，可能是因为使用了含有gimel 5的替代轻链。这里提供的数据是关于> 100个含有V κ J κ的转录物和类似或等于180个基因组V κ基因，以表明>90%的前-免疫库由IGKV 2基因的三个亚家族和五个J κ区段之一产生。kappa基因座包含至少5个亚家族的≥ 50个IGKV 2基因和一个未确定但可能相等数量的IGKV 1基因。猪IGKV 1和IGKV 2基因与它们的人类对应物共享87%的序列相似性，并且J κ 1至J κ 5与绵羊、马、人和小鼠中的那些共享序列和组织同源性。猪有一个单一的C κ基因，这些发现与啮齿类和灵长类动物的结果相反，但让人想起那些在猪免疫前的重链库，因为它是使用相对有限数量的基因片段产生的。这些有限的免疫前库可能反映了胎儿对母体因素和环境抗原的最小暴露。猪免疫学的意义，表征免疫前的剧目进行了讨论。(c)2005 Elsevier B. V.保留所有权利。
Combinatorial diversity is highly restricted during formation of the pre-immune heavy chain repertoire of swine, raising the question of whether the same is true for the pre-immune light chain repertoire. Before addressing this question, we first used competitive PCR to show that kappa and lambda light chains in swine are equally expressed in mature B cells similar to the situation in humans but alike that in other studied Ungulates. This justified efforts to examine the repertoire of both light chain, types. These studies also revealed that lambda is preferentially expressed at sites of B cells lymphogenesis, perhaps because of the use of a surrogate light chain containing gimel 5.Data are presented here on > 100 V kappa J kappa-Containing transcripts and similar or equal to 180 genomic V kappa genes to show that >90% of the pre-immune repertoire is generated from three subfamilies of IGKV2 genes and one of five J kappa segments. The kappa locus contains >= 50 IGKV2 genes belonging to at least five subfamilies and an undetermined but perhaps equal number of IGKV1 genes. The porcine IGKV1 and IGKV2 genes share 87% sequence similarity with their human counterparts and J kappa 1 through J kappa 5 share sequence and organizational homology with those in sheep, horse, human and mouse. Swine have a single C kappa gene.These findings contrast with those from rodents and primates but are reminiscent of those on the pre-immune heavy chain repertoire of swine in that it is generated using a relatively restricted number of gene segments. These restricted pre-immune repertoires may reflect the minimal exposure of the fetus to materna factors and environmental antigens. The significance for swine immunology of characterizing the pre-immune repertoire is discussed. (c) 2005 Elsevier B.V. All rights reserved.