Identification and characterization of a novel benzo[a] pyrene-derived DNA adduct
Identification and characterization of a novel benzo[a] pyrene-derived DNA adduct
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DOI：
10.1006/bbrc.2000.4161
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发表时间：
2001-02-23
影响因子：
3.1
通讯作者：
Gupta, RC
中科院分区：
文献类型：
--
作者：
Fang, AH;Smith, WA;Gupta, RC
本研究的目的是合成和鉴定一种新的苯并[a]芘(BP)衍生的脱氧核糖核酸加合物。迄今为止，大多数研究都集中在BP生物活化后产生的主要加合物N-2-[10β(7β，8a，9a-trihydroxy-7，8，9，10-tetrahydrobenzo[a]pyrene)yl]-deoxyguanosine(抗BPDE-DG))，然而，BP在生物活化后也会形成第二加合物，推测这是9-OH-BP进一步代谢的结果，以确定这第二反应途径。最终的DNA结合物种，以及所涉及的DNA碱基，我们已经合成并表征了一种DG衍生的DNA加合物，该加合物来自9-OH-BP在大鼠肝微粒体存在下的进一步生物激活，使用P-32后标记分析和毛细管电泳质谱(CE-MS)对加合物的核苷酸进行了分析，(C)2001学术出版社。
The aim of this study was to generate and identify a novel benzo[a]pyrene (BP)-derived DNA adduct found both in vitro and in vivo, To date, the majority of studies have focused on N-2-[10 beta (7 beta ,8a,9a-trihydroxy-7,8,9,10-tetrahydrobenzo[a]pyrene)yl]-deoxyguanosine (anti-BPDE-dG), the major adduct generated following bioactivation of BP, However, a second adduct is also formed following bioactivation of BP which has been speculated to result from further metabolism of 9-OH-BP, In order to identify this second reaction pathway, the ultimate DNA binding species, and the DNA base involved, we have synthesized and characterized a dG-derived DNA adduct arising from further bioactivation of 9-OH-BP in the presence of rat liver microsomes, Analysis of the adducted nucleotides was conducted using both the P-32-postlabeling assay and capillary electrophoresis-mass spectrometry (CE-MS), (C) 2001 Academic Press.