Double-Enzymes-Mediated Bioluminescent Sensor for Quantitative and Ultrasensitive Point-of-Care Testing
Double-Enzymes-Mediated Bioluminescent Sensor for Quantitative and Ultrasensitive Point-of-Care Testing
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用于定量和超灵敏即时检测的双酶介导的生物发光传感器
DOI：
10.1021/acs.analchem.7b00239
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发表时间：
2017-05-16
影响因子：
7.4
通讯作者：
Jiang, Xingyu
中科院分区：
文献类型：
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作者：
Chen, Yiping;Xianyu, Yunlei;Jiang, Xingyu
我们报道了一种超灵敏、定量和快速的生物发光免疫传感器(ABS)，用于使用包括碱性磷酸酶(ALP)和荧光素酶在内的双酶对临床样本中的疾病生物标记物进行点检测(POCT)。在生物标志物的存在下，附着在免疫纳米复合体表面的碱性磷酸酶(ALP)去磷酸化三磷酸腺苷(ATP)，从而抑制ATP荧光素荧光素酶的生物发光反应。ATP的高灵敏响应(皮摩尔水平)允许通过ALP和荧光素酶催化的反应有效地改变生物发光强度来超灵敏地检测生物标记物，这可以由便携式ATP检测器定量测定。这种ABS符合POCT的标准，即用最少的设备(便携式检测器)快速(不到1小时)进行灵敏(生物标志物的毫摩尔水平)和定量测量。
We report an ultrasensitive, quantitative, and rapid bioluminescent immunosensor (ABS) for point-of-care testing (POCT) of the disease biomarker in clinical samples using double enzymes including alkaline phosphatase (ALP) and luciferase. In the presence of the biomarker, the ALP attached on the surface of immuno-nanocomplex dephosphorylates adenine triphosphate (ATP), subsequently inhibiting the ATP luciferin luciferase bioluminescent reaction. The highly sensitive response of ATP (picomolar level) allows for ultrasensitive detection of biomarker via the effective change of the bioluminescence intensity through ALP- and luciferase-catalyzed reactions, which can be quantitatively determined by a portable ATP detector. This ABS fulfills the criteria for POCT that performs sensitive (femtomolar level of biomarkers) and quantitative measurement quickly (less than 1 h) with minimal equipment (portable detector).