Amine uptake into intact mast cell granules in vitro.
Amine uptake into intact mast cell granules in vitro.
复制标题
体外完整肥大细胞颗粒中的胺摄取。
DOI：
10.1021/bi00368a068
复制
发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Lagunoff,D
中科院分区：
文献类型：
--
作者：
Ludowyke,RI;Lagunoff,D
Materials and MethodsMaterials were obtained from the indicated sources as follows: bovine albumin (Path-O-Cyte 4) from Miles Laboratories Inc.(Elkhart, IN); Percoll from Pharmacia Fine Chemicals (Piscataway, NJ); tyramine hydrochloride from Calbiochem (La Jolla, CA);[3H]-histamine (5.8 Ci/mmol) from New England Nuclear (Boston, MA);[3H]-5-hydroxytryptamine creatinine sulfate (12.3 Ci/mmol) fromAmersham (Arlington Heights, IL);[3H] dopamine (53 Ci/mmol) from Research Products Int.(Mt. Prospect, IL); dopamine hydrochloride, histamine dehydrochloride, 5-hydroxytryptamine creatinine sulfate, and ATP (sodium salt, grade 1, crystalline) from Sigma Chemical Co.(St. Louis, MO). Mast cells were obtained from male Sprague-Dawley rats (400-500 g)(Sasco, Omaha, NE) by peritoneal lavage and collection as previously described (Lagunoff, 1975). A pop-ulation of 95-98% puremast cells was achieved following centrifugation at 225g, for 15 min through a Percoll gradient (7 mL of Percoll with 1.0 mL of 10 X concentrated salts solution containing 1.54 M NaCl, 27 mM KC1, and 9 mMCaCl2, plus 0.5% albumin, adjusted to pH 7.2 with 0.1 M KH2P04 and final volumemade up to 10.0 mL). The purified mast cells were washed twice in balancedsalts solution con-taining albumin (BSSA; diluted salts as above were adjusted to pH 7.2 with 0.1 M phosphate buffer, and 0.5% albumin was added) and resuspended in 2.0 mL of BSSA for sonication [(2-3) X 106 mast cells/mL]. Sonication was effected in
