Characterization of muscarinic acetylcholine receptors in human labial salivary glands.
Characterization of muscarinic acetylcholine receptors in human labial salivary glands.
复制标题
人唇唾液腺毒蕈碱乙酰胆碱受体的表征。
DOI：
10.1016/0014-2999(90)90032-2
复制
发表时间：
1990
影响因子：
5
通讯作者：
Yamamura,HI
中科院分区：
文献类型：
--
作者：
Mei,L;Roeske,WR;Izutsu,KT;Yamamura,HI
用（−）-[3 H]MQNB研究了人类唇唾液腺中的毒蕈碱乙酰胆碱受体。放射性配体以150 pM的Kd值结合。毒蕈碱受体的密度为220 fmol/mg蛋白。大多数毒蕈碱拮抗剂结合到该组织中的毒蕈碱受体的同质群体。然而，哌仑西平的抑制曲线具有0.57的Hill斜率，并且可以通过双位点模型进行分析。提示人唇腺M受体为M_1和M_3的混合型。
Muscarinic acetylcholine receptors were studied in human labial salivary glands with (−)-[3H]MQNB. The radioligand bound with a Kdvalue of 150 pM. The density of muscarinic receptors was 220 fmol/mg protein. Most muscarinic antagonists bound to a homogenous population of muscarinic receptors in this tissue. However, the inhibition curves of pirenzepine had a Hill slope of 0.57 and could be analyzed by a two site model. These results suggest that the muscarinic receptors in human labial salivary glands are a mixture of M1and M3types.