hTERT expression in human breast cancer and non-cancerous breast tissue: correlation with tumour stage and c-Myc expression
hTERT expression in human breast cancer and non-cancerous breast tissue: correlation with tumour stage and c-Myc expression
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DOI：
10.1023/a:1021849217054
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发表时间：
2003-02-01
影响因子：
3.8
通讯作者：
Mokbel, K
中科院分区：
文献类型：
--
作者：
Kirkpatrick, KL;Ogunkolade, W;Mokbel, K
端粒酶是一种核糖核蛋白酶，在细胞分裂后合成端粒并维持染色体长度和稳定性，从而导致细胞永生化。hTERT（human telomerase reverse transcriptase）基因是端粒酶再激活的限速因子。本研究旨在定量检测人乳腺癌、癌旁组织（ANCT）和乳腺良性病变组织中hTERT mRNA的表达，探讨hTERT与乳腺癌临床病理特征的关系，以及c-Myc与hTERT表达的关系。从49例乳腺癌、46例匹配的ANCT和8例纤维腺瘤中提取RNA。RT-PCR和Taqman方法检测hTERT和c-Myc mRNA表达。hTERTmRNA在所有癌组织和大多数ANCT标本中均存在，癌组织中的表达水平远高于ANCT。肿瘤与ANCT中hTERTmRNA的比值为2011（95%可信区间373-10,853，P < 0.0001）。肿瘤hTERT表达与患者年龄、肿瘤大小、分级、淋巴结转移、雌激素受体（ER）阳性、淋巴管（LVI）或c-Myc表达之间无显著相关性。然而，在肿瘤中hTERT表达和孕激素受体（PR）状态之间存在微弱但显著的负相关性（p = 0.04）。hTERTmRNA在癌中的表达（中位数= 2.61 × 10（6））也显著高于纤维腺瘤（中位数= 424）。我们的结论是，hTERTmRNA的表达显着高于在人类乳腺癌比非癌乳腺组织，这表明hTERT在乳腺癌诊断中具有潜在的作用。肿瘤中的hTERT mRNA水平似乎与患者的年龄或晚期肿瘤阶段无关。乳腺癌中hTERT mRNA表达与c-Myc mRNA表达无相关性。
Telomerase is a ribonucleoprotein enzyme that synthesises telomeres after cell division and maintains chromosomal length and stability thus leading to cellular immortalisation. hTERT (human telomerase reverse transcriptase) gene is the rate-limiting determinant of telomerase reactivation. The present study aims to quantitatively measure the expression of hTERT mRNA in human breast cancer, adjacent non-cancerous tissue (ANCT) and benign breast lesions, examine the association between hTERT and the clinicopathological characteristics of the cancer specimens and to explore the relationship between c-Myc and hTERT expressions. RNA was extracted from 49 breast carcinomas, 46 matched ANCT, and eight fibroadenomas. hTERT and c-Myc mRNA expressions were estimated by reverse transcriptase-PCR (RT-PCR) and Taqman methodology. hTERT mRNA was present in all of the cancerous and most of ANCT specimens with levels being much higher in the cancerous tissue than in ANCT. The ratio of hTERT mRNA in tumour to that in ANCT was 2011 (95% confidence interval 373-10,853, P < 0.0001). There was no significant association between tumour hTERT expression and patient's age, tumour size, grade, nodal metastasis, estrogen receptor (ER) positivity, lymphovascular (LVI) or c-Myc expression. However, there was a weak but significant negative correlation between hTERT expression and progesterone receptor (PR) status (p = 0.04) in tumours. hTERT mRNA expression was also significantly higher in carcinomas (median = 2.61 x 10(6)) than in fibroadenomas (median = 424). We conclude that hTERT mRNA expression is significantly higher in human breast cancer than in non-cancerous breast tissue suggesting that hTERT has a potential role in breast cancer diagnosis. The hTERT mRNA levels in tumour do not seem to be associated with the patient's age or advanced tumour stage. Furthermore, hTERT mRNA expression does not correlate with c-Myc mRNA expression in breast cancer.