Leptin in human reproduction: Serum leptin levels in pregnant and lactating women
Leptin in human reproduction: Serum leptin levels in pregnant and lactating women
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DOI：
10.1210/jc.82.2.585
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发表时间：
1997-02-01
影响因子：
5.8
通讯作者：
Nicolson, MA
中科院分区：
文献类型：
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作者：
Butte, NF;Hopkinson, JM;Nicolson, MA
ob/ob雌性小鼠的实验表明，注射瘦素不仅可以减轻体重和脂肪量，还可以恢复生育能力和部分哺乳。为了探讨生育妇女ob基因表达的调节因素，我们测定了65名妇女在妊娠36周、产后3个月和6个月时的血清瘦素、体脂、能量消耗和产奶量。血清瘦素采用固相双抗体夹心酶联免疫法测定，血清胰岛素和PRL采用固相I-125放射免疫法测定。采用氘稀释法测定总水分，采用氢密度法测定体体积，采用双能X线吸收法测定骨密度，以估计体脂。妊娠36周时每单位脂肪量的血清瘦素显著高于产后3个月和6个月（1.25 vs 0.75，0.73 ng）。mL（-1）。kg（-1））。产后瘦素正常化不仅与体重和脂肪量的变化有关，还与血清胰岛素的变化有关。瘦素在哺乳期和非哺乳期妇女之间没有差异。瘦素可能通过其对血清PRL的负面影响间接影响产奶量。校正去脂量和脂肪量后，能量消耗率与瘦素无显著相关性。我们的研究结果提供了证据，其他因素而不是脂肪量单独调节血清瘦素在生殖妇女。
Experiments in ob/ob female mice demonstrated that leptin injections not only reduced weight and fat mass, but also restored fertility and partial lactation. To explore factors regulating ob gene expression in reproductive women, we measured serum leptin, body fat, energy expenditure, and milk production in 65 women at 36 weeks of gestation, and at 3 and 6 months postpartum. Serum leptin was measured by solid-phase sandwich enzyme immunoassay, and serum insulin and PRL by solid-phase I-125 RIA. Total body water by deuterium dilution, body volume by hydrodensitometry, and bone density by dual-energy x-ray absorptiometry were used to estimate body fat. Serum leptin per unit fat mass was significantly higher at 36 weeks of pregnancy than at 3 and 6 months postpartum (1.25 vs. 0.75, 0.73 ng . mL(-1). kg(-1)). Postpartum normalization of leptin was associated with changes not only in weight and fat mass, but also serum insulin. Leptin was not different between lactating and nonlactating women. Leptin may have affected milk production indirectly through its negative effect on serum PRL. Adjusted for fat-free mass and fat mass, rates of energy expenditure were not significantly correlated with leptin. Our results provide evidence that factors other than fat mass alone modulate serum leptin in reproductive women.