Synaptic plasticity in hippocampal CA1 neurons and learning behavior in transient ischemia-loaded gerbils
Synaptic plasticity in hippocampal CA1 neurons and learning behavior in transient ischemia-loaded gerbils
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DOI：
10.2220/biomedres.34.75
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发表时间：
2013-04-01
影响因子：
1.2
通讯作者：
Fujii, Satoshi
中科院分区：
文献类型：
--
作者：
Li, Jianmin;Sasaki, Hiroshi;Fujii, Satoshi
我们研究了沙土鼠双侧颈总动脉结扎5min和10min造成的短暂性脑缺血后，海马CA1区神经元突触可塑性和空间学习行为的变化。短暂性脑缺血后引出场反应的刺激阈值与对照组没有差异，但突触反应的幅度显著降低，这可能是由于观察到的神经元丢失所致。脑缺血10min后的长时程增强(LTP)和去增强以破伤风前或前低频刺激值的百分比表示，与对照组几乎相同，尽管LTP和去增强的实际幅度低于对照组。沙土鼠在脑缺血10天后开始进行空间学习测试，而在相同的脑缺血20天后开始进行空间学习测试时，沙土鼠在10 mm的脑缺血后表现出空间学习障碍。这些结果提示，10 mm脑缺血后10天海马CA1区神经元电生理特性的改变可能是沙土鼠空间学习能力受损的原因之一，而CA1区外区域可能参与了脑缺血20天后空间学习能力的恢复。
We studied the synaptic plasticity of hippocampal CA1 neurons and spatial learning behavior in gerbils that had been loaded with a transient cerebral ischemia caused by 5 min or 10 min occlusion of the bilateral carotid arteries. The stimulus threshold to elicit the field responses after a transient cerebral ischemia was not different from that in controls, but there was a significant decrease in the magnitude of synaptic responses, which might result from the observed loss of neurons. Long-term potentiation (LTP) and depotentiation after a 10 min cerebral ischemia expressed as a percentage of the pre-tetanus or pre-low frequency stimulation value were almost the same as those in controls, although the actual magnitude of the LTP and depotentiation was lower than in controls. Gerbils that were loaded with a 10 mm cerebral ischemia showed impairment in a spatial learning test when this was started 10 days after the cerebral ischemia, but not when it was started 20 days after the same cerebral ischemia. These results suggest that the changes in electrophysiological properties of hippocampal CA1 neurons seen at 10 days after a 10 mm cerebral ischemia contribute to the impairment of spatial learning of gerbils seen at this time, and that the extra-CA1 regions might be involved in the recovery of spatial learning seen at 20 days after cerebral ischemia.