Anti-Aging Effects of Hydrophobic and Hydrophilic Components From Immature Pear Fruits Extract
Anti-Aging Effects of Hydrophobic and Hydrophilic Components From Immature Pear Fruits Extract
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未成熟梨果实提取物的疏水和亲水性成分的抗衰老作用
DOI：
10.1093/geroni/igaa057.427
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发表时间：
2020-12-16
影响因子：
7
通讯作者：
Fujita KI
中科院分区：
文献类型：
--
作者：
Ogita A;Murata W;Yamauchi K;Sakai A;Yamaguchi Y;Tanaka T;Fujita KI
细胞衰老是由于衰老导致的细胞功能的下降，导致生理功能的逐渐丧失，并诱发一些慢性疾病，从而对人类的生活质量产生负面影响。对细胞衰老过程的干预可能会减少这些发病率，并延缓与年龄有关的疾病的进展，从而有助于延长人类寿命。芽殖酵母（Saccharomyces cerevisiae）是一种模型系统，可以为衰老的遗传学和分子生物学提供重要见解，并且是研究哺乳动物细胞的合适细胞模型。在2019年的GSA会议上，我们发现酵母细胞寿命的延长是通过添加未成熟梨果实提取物（iPE）诱导的。在这项研究中，我们重点研究了iPE的亲水性（WiPE）和疏水性（OiPE）组分对酵母细胞及其基因的抗衰老作用，以及它们在延长人类寿命方面的可能应用。采用小鼠时间寿命法研究iPE的抗衰老作用。酿酒酵母细胞在用1%（v/v）的WiPE和OiPE两者处理的那些中，酵母的时间寿命显著延长。通过RT-PCR检测调节细胞衰老的sirtuin相关基因的表达。有趣的是，发现基因表达仅在WiPE处理的细胞中显著增加。结果表明，iPE中不同极性的成分通过不同的机制发挥抗衰老作用。目前正在研究鉴定iPE中的有用成分以及应用于哺乳动物细胞的可能性。
Cellular senescence, the decline of cellular function due to aging, causes gradual loss of physiological functions and induces some chronic diseases, which negatively affect the quality of human life. Intervention in the cellular senescence process may reduce these incidences and delay the progression of age-related diseases, thereby contributing to the longevity of human lifespan. The budding yeast, Saccharomyces cerevisiae, is a model system that can provide significant insights into the genetics and molecular biology of senescence and is a suitable cellular model for research on mammalian cells. In the 2019 GSA meeting, we had revealed that the prolongation of yeast cell lifespan was induced by the addition of immature pear fruits extracts (iPE). In this study, we have focused on investigating the anti-senescence effects of hydrophilic (WiPE) and hydrophobic (OiPE) components of iPE on yeast cells and their genes and their possible application in extending human lifespan. The anti-aging effects of iPE were investigated using a chronological lifespan assay on S. cerevisiae cells. The chronological lifespan of the yeast was significantly extended in those treated with both WiPE and OiPE at 1% (v/v). The expression of sirtuin-related genes, which regulate cellular senescence, was examined by RT-PCR. Interestingly, gene expression was found to be significantly increased only in WiPE treated cells. The results suggested that the different polarity components from iPE exhibited anti-aging effects on the cells via different mechanisms. Research on the identification of useful components in iPE and the possibility of application to mammalian cells is ongoing.