Functional characterization of two human MutY homolog (hMYH) missense mutations (R227W and V232F) that lie within the putative hMSH6 binding domain and are associated with hMYH polyposis.
Functional characterization of two human MutY homolog (hMYH) missense mutations (R227W and V232F) that lie within the putative hMSH6 binding domain and are associated with hMYH polyposis.
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DOI：
10.1093/nar/gki209
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发表时间：
2005
影响因子：
14.9
通讯作者：
Lu AL
中科院分区：
文献类型：
--
作者：
Bai H;Jones S;Guan X;Wilson TM;Sampson JR;Cheadle JP;Lu AL
The base excision repair DNA glycosylase MutY homolog (MYH) is responsible for removing adenines misincorporated into DNA opposite guanine or 7,8-dihydro-8-oxo-guanine (8-oxoG), thereby preventing G:C to T:A mutations. Biallelic germline mutations in the human MYH gene predispose individuals to multiple colorectal adenomas and carcinoma. We have recently demonstrated that hMYH interacts with the mismatch repair protein hMSH6, and that the hMSH2/hMSH6 (hMutSα) heterodimer stimulates hMYH activity. Here, we characterize the functional effect of two missense mutations (R227W and V232F) associated with hMYH polyposis that lie within, or adjacent to, the putative hMSH6 binding domain. Neither missense mutation affects the physical interaction between hMYH and hMSH6. However, hMYH(R227W) has a severe defect in A/8-oxoG binding and glycosylase activities, while hMYH(V232F) has reduced A/8-oxoG binding and glycosylase activities. The glycosylase activity of the V232F mutant can be partially stimulated by hMutSα but cannot be restored to the wild-type level. Both mutants also fail to complement mutY-deficiency in Escherichia coli. These data define the pathogenic mechanisms underlying two further hMYH polyposis-associated mutations.
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影响因子：
2.9
作者：
MICHAELS, ML;TCHOU, J;MILLER, JH
通讯作者：
MILLER, JH
影响因子：
64.8
作者：
Fromme, JC;Banerjee, A;Verdine, GL
通讯作者：
Verdine, GL
影响因子：
2.9
作者：
Lu, AL;Wright, PM
通讯作者：
Wright, PM
DOI：
10.1073/pnas.90.3.1122
发表时间：
1993-02-01
影响因子：
11.1
作者：
MORIYA, M
通讯作者：
MORIYA, M
影响因子：
3.5
作者：
Peltomäki, P
通讯作者：
Peltomäki, P