Measuring Nuclear Mechanics with Atomic Force Microscopy.
Measuring Nuclear Mechanics with Atomic Force Microscopy.
复制标题
用原子力显微镜测量核力学。
DOI：
10.1007/978-1-0716-2221-6_13
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Dos Santos Á
中科院分区：
文献类型：
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作者：
Dos Santos Á
原子力显微镜是在微米和纳米尺度上绘制材料的形貌和力学性能的理想工具。在这里，我们描述了它在测量和分析活细胞中哺乳动物细胞核的力学，特别是有效的杨氏弹性模数E*方面的应用。我们提出了三种方法，使这些机制能够在不同的条件下进行探索。这包括完全贴壁的细胞，最初贴壁的细胞缺乏已建立的细胞骨架，以及纯化的细胞核来研究它们的孤立反应。
Atomic force microscopy is an ideal tool to map topography and mechanical properties of materials on the micro- and nanoscale. Here, we describe its application to measure and analyze the mechanics, in particular the effective Young’s elastic modulusE*of the mammalian nucleus in live cells. We present three approaches which enable the mechanics to be probed under varying conditions. This includes fully adhered cells, initially adhered cells which lack an established cytoskeleton, and purified nuclei to study their isolated response.