Rab11 is essential for fertility in Drosophila
Rab11 is essential for fertility in Drosophila
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DOI：
10.1016/j.cellbi.2008.04.002
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发表时间：
2008-09-01
影响因子：
3.9
通讯作者：
Roy, Jagat K.
中科院分区：
文献类型：
--
作者：
Tiwari, Anand K.;Roy, Jagat K.
Rab 11是一种参与囊泡运输的小GTP结合蛋白，已成为调节果蝇发育和分化过程中各种细胞事件的关键参与者。在我们早期的研究中，建立了一个P-插入系Rab 11（mo），作为Rab 11基因的一个新的亚型等位基因，表现出退化的眼睛表型，刚毛异常和不育。我们发现Rab 11在整个睾丸中表达，在分泌细胞中表达更明显，在卵巢中定位于后极。Rab 11（mo）雄性和雌性不育。雄性不育归因于精子个体化过程中的缺陷，而在雌性中，由于Rab 11的丢失/错误定位，细胞骨架破坏和后部定位蛋白Vasa的减少/丢失可能是不育的原因。在pCaSpeR 4-Rab 11/+; Rab 11（mo）/Rab 11（mo）卵室中恢复了生殖力以及Rab 11和Vasa的后部定位或细胞骨架完整性，证实了在这些事件中Rab 11的需要。(C)2008年由Elsevier Ltd代表国际细胞生物学联合会出版。
Rab11, a small GTP binding protein involved in vesicular trafficking, has emerged as a key player in regulating various cellular events during Drosophila development and differentiation. In our earlier study a P-insertion line, Rab11(mo), was established as a new hypomorphic allele of Rab11 gene, showing degenerated eye phenotype, bristle abnormalities and sterility. We show here that Rab11 is expressed in the entire testis, more prominently in the secretory cells, and in ovary it is localized at the posterior pole. Rab11(mo) males and females are sterile. The sterility in males has been attributed to defects in the sperm individualization process, while in females, cytoskeleton disruption and reduction/loss of the posteriorly localized protein, Vasa, as a consequence of loss/mislocalization of Rab11 might be the cause of sterility. Fertility as well as the posterior localization of Rab11 and Vasa or cytoskeleton integrity was restored in pCaSpeR4-Rab11/+; Rab11(mo)/Rab11(mo) egg chambers, confirming the requirement of Rab11 in these events. (C) 2008 Published by Elsevier Ltd on behalf of International Federation for Cell Biology.