MEASUREMENT OF MICROBIAL BIOMASS PHOSPHORUS IN SOIL
MEASUREMENT OF MICROBIAL BIOMASS PHOSPHORUS IN SOIL
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DOI：
10.1016/0038-0717(82)90001-3
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发表时间：
1982-01-01
影响因子：
9.7
通讯作者：
JENKINSON, DS
中科院分区：
文献类型：
--
作者：
BROOKES, PC;POWLSON, DS;JENKINSON, DS
本文介绍了一种测定土壤微生物中磷含量的方法，并讨论了该方法所依据的假设。生物量P的计算方法是用0.5 M NaHCO 3（pH 8.5）从用CHCl 3熏蒸的新鲜土壤中提取的无机P（Pi）的量与从未熏蒸土壤中提取的量之间的差异。在熏蒸和提取过程中，一些CHCl 3释放的Pi被土壤吸附：通过在提取过程中加入已知量的Pi并校正回收率，可以近似地允许这一点。大部分的磷释放是在无机形式和比例增加熏蒸持续时间。非微生物磷受熏蒸影响很小（如果有的话）。微生物生物量P由CHCl 3释放的Pi除以0.4计算，即，假设生物质中40%的P被CHCl 3提取为Pi。生物量P的测量必须在新鲜土壤中进行; CHCl 3在风干土壤中释放的P要少得多。
A method for measuring the amount of P held in soil microorganisms (biomass P) is described and the assumptions on which it is based are discussed. Biomass P in calculated from the difference between the amount of inorganic P (Pi) extracted by 0.5 M NaHCO3 (pH 8.5) from fresh soil fumigated with CHCl3 and the amount extracted from unfumigated soil. Some CHCl3-released Pi is sorbed by soil during fumigation and extraction: an approximate allowance for this is made by incorporating a known quantity of Pi during extraction and correcting for recovery. Most of the P released is in inorganic form and the proportion increases with duration of fumigation. Non-microbial P is little, if at all, affected by fumigation. Microbial biomass P is calculated from CHCl3-released Pi by dividing by 0.4, i.e., by assuming that 40% of the P in the biomass is rendered extractable as Pi by CHCl3. Measurements of biomass P must be done in fresh soil; CHCl3 releases much less P in air-dry soil.