Discrimination of wild and cultured Japanese eels based on otolith stable isotope ratios
Discrimination of wild and cultured Japanese eels based on otolith stable isotope ratios
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基于耳石稳定同位素比值区分野生和养殖日本鳗鱼
DOI：
10.1093/icesjms/fsx173
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发表时间：
2017
影响因子：
3.3
通讯作者：
Handling editor: Caroline Durif
中科院分区：
文献类型：
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作者：
Kaifu Kenzo;Itakura Hikaru;Amano Yosuke;Shirai Kotaro;Yokouchi Kazuki;Wakiya Ryoshiro;Murakami-Sugihara Naoko;Washitani Izumi;Yada Takashi;Handling editor: Caroline Durif
我们成功地在没有人工标记的情况下区分了野生和养殖鳗鱼。为了恢复鳗鱼的种群数量，放养在欧洲联盟(EU)和日本得到了广泛的开展，尽管放养的净效益尚不清楚。为了评估放养鳗鱼对恢复种群的效果，应跟踪放养的鳗鱼的生存、生长、逃逸和繁殖情况。在这项研究中，我们探索了使用耳石氧和碳稳定同位素比率作为区分野生和养殖鳗鱼的自然标记的潜力，因为养殖的鳗鱼经常被放养到自然河流和湖泊中。以野生鳗鱼(n= 95)和养殖鳗鱼(n= 314)为训练数据集，建立了判别模型。基于留一法交叉验证的准确率估计结果为96.8%。然后，我们测试了该模型对重新放养的鳗鱼(n= 20)的适用性；100.0%的鳗鱼被成功识别为养殖的鳗鱼，这表明这些鳗鱼在放养之前在水产养殖池塘中度过了它们的早期大陆期。这种方法可以广泛应用于评估放养鳗鱼的效果，方法是确定从河流、沿海地区或产卵场捕获的鳗鱼的放养比例。
We successfully discriminated wild and cultured anguillid eels without artificial tagging. For the purpose of population restoration of anguillid eels, stocking has been widely conducted in the European Union (EU) and Japan, although the net benefits of stocking remain unclear. To evaluate the effectiveness of eel stocking for population restoration, the survival, growth, escapement, and reproduction of stocked eels should be tracked. In this study, we explored the potential of using otolith oxygen and carbon stable isotope ratios as a natural tag to discriminate between wild and cultured eels, because cultured eels are often stocked into natural rivers and lakes. A discrimination model was developed based on wild (n= 95) and cultured (n= 314) Japanese eels as a training dataset. The results of accuracy estimation based on leave-one-out cross-validation were 96.8%. We then tested the applicability of the model to stocked–recaptured eels (n= 20); 100.0% were successfully identified as cultured eels, indicating that these eels spend their early continental phase in aquaculture ponds before stocking. This method could be widely applied to assess the effectiveness of eel stocking by determining the proportion of stocked eels among those captured from rivers, coastal areas, or spawning grounds.