A novel culture device for the evaluation of three‐dimensional extracellular matrix materials
A novel culture device for the evaluation of three‐dimensional extracellular matrix materials
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一种用于评估三维细胞外基质材料的新型培养装置
DOI：
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发表时间：
2014
影响因子：
3.3
通讯作者：
A. Lichtenberg
中科院分区：
文献类型：
--
作者：
P. Akhyari;Heiko Ziegler;P. Gwanmesia;M. Barth;Soeren Schilp;J. Huelsmann;S. Hoffmann;J. Bosch;G. Kögler;A. Lichtenberg
三维（3D）细胞外基质（ECM）中的细胞-基质相互作用在活组织中具有根本的重要性，并且它们在生物人工结构中的体外重建代表了当代组织工程概念的核心目标。为了在高度受控的条件下详细分析细胞-基质相互作用，我们开发了一种新型ECM评估培养装置（EECD），该装置允许精确定义3D ECM支架的表面接种，而不管其自然几何形状如何。在组织工程心脏瓣膜的背景下评价EECD的有效性。将清洁剂脱细胞的肺尖固定在EECD中，并与内皮细胞（EC）一起接种，以研究3、24、48和96 h后EC在3D ECM上的粘附、形态和功能。标准EC单层用作对照。通过激光扫描显微镜（LSM）证实了活EC对3D ECM的排他性顶面接种，导致96 h后ECM融合再内皮化。如通过MTS测定和蛋白质印迹分析（内皮型一氧化氮合酶，血管性血友病因子）所证明的，细胞活力和蛋白质表达随时间保持在维持水平。总之，EECD被证明是一种非常有效的系统，用于控制再增殖和体外分析3D ECM中的细胞-ECM相互作用。版权所有© 2012约翰威利父子有限公司.
Cell–matrix interactions in a three‐dimensional (3D) extracellular matrix (ECM) are of fundamental importance in living tissue, and their in vitro reconstruction in bioartificial structures represents a core target of contemporary tissue engineering concepts. For a detailed analysis of cell–matrix interaction under highly controlled conditions, we developed a novel ECM evaluation culture device (EECD) that allows for a precisely defined surface‐seeding of 3D ECM scaffolds, irrespective of their natural geometry. The effectiveness of EECD was evaluated in the context of heart valve tissue engineering. Detergent decellularized pulmonary cusps were mounted in EECD and seeded with endothelial cells (ECs) to study EC adhesion, morphology and function on a 3D ECM after 3, 24, 48 and 96 h. Standard EC monolayers served as controls. Exclusive top‐surface‐seeding of 3D ECM by viable ECs was demonstrated by laser scanning microscopy (LSM), resulting in a confluent re‐endothelialization of the ECM after 96 h. Cell viability and protein expression, as demonstrated by MTS assay and western blot analysis (endothelial nitric oxide synthase, von Willebrand factor), were preserved at maintained levels over time. In conclusion, EECD proves as a highly effective system for a controlled repopulation and in vitro analysis of cell–ECM interactions in 3D ECM. Copyright © 2012 John Wiley & Sons, Ltd.
DOI：
10.1016/j.matbio.2009.04.005
发表时间：
2009-06
期刊：
Matrix biology : journal of the International Society for Matrix Biology
影响因子：
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作者：
Patricia A. Soucy;L. Romer
通讯作者：
Patricia A. Soucy;L. Romer
小口径血管移植物的体外内皮化。
影响因子：
3.8
作者：
Anderson,JS;Price,TM;Hanson,SR;Harker,LA
通讯作者：
Harker,LA