Rapid Susceptibility Testing Methods
Rapid Susceptibility Testing Methods
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DOI：
10.1016/j.cll.2019.04.001
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发表时间：
2019-09-01
影响因子：
1.7
通讯作者：
Kirby, James E.
中科院分区：
文献类型：
--
作者：
Smith, Kenneth P.;Kirby, James E.
随着抗菌素耐药性的出现，需要快速抗微生物药敏试验（AST）提供早期明确的治疗指导，以优化患者的预后。基因型方法快速，但只能识别已知抗性元素的一个子集。表型方法确定临床预测的最低抑制浓度，包括非常敏感的光学和生物物理方法，以检测病原体对抗生素的复制或生理反应的变化。为了充分实现快速AST的潜力，必须将结果与强大的决策支持解决方案联系起来，这些解决方案将实时实施治疗变化。
With emerging antimicrobial resistance, rapid antimicrobial susceptibility testing (AST) is needed to provide early definitive therapeutic guidance to optimize patient outcome. Genotypic methods are fast, but can identify only a subset of known resistance elements. Phenotypic methods determine clinically predictive minimal inhibitory concentrations and include very sensitive optical and biophysical methods to detect changes in replication or physiology of pathogens in response to antibiotics. For the potential of rapid AST to be fully realized, results must be linked with robust decision support solutions that will implement therapeutic changes in real time.