COMPLEXITY OF HUMAN T-CELL ANTIGEN RECEPTOR BETA-CHAIN CONSTANT-REGION AND VARIABLE-REGION GENES
COMPLEXITY OF HUMAN T-CELL ANTIGEN RECEPTOR BETA-CHAIN CONSTANT-REGION AND VARIABLE-REGION GENES
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DOI：
10.1038/312541a0
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发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
RABBITTS, TH
中科院分区：
文献类型：
--
作者：
SIMS, JE;TUNNACLIFFE, A;RABBITTS, TH
脊椎动物的免疫系统通过两种类型的效应细胞B和T细胞发挥功能，这两种细胞能够进行抗原特异性识别。免疫球蛋白作为B细胞上的抗原受体，与T细胞受体不同，其基因结构已得到很好的表征。最近，已经描述了被认为对应于人和小鼠T细胞受体的β链位点2 - 4的cDNA克隆。推定的β链克隆特异性检测T细胞DNA中的基因重排，并显示与免疫球蛋白轻链的同源性。T细胞β链基因系统与免疫球蛋白基因的相似性已通过最近对可变区和恒定区基因片段以及连接片段和推定多样性片段的观察得到进一步证明5，6。本文报道了编码人T细胞受体β链基因的cDNA和基因组克隆的特征。有两个恒定区基因（Cβ1和C β2），每个基因都能重排并表达为RNA。基因排列与小鼠β-链基因相似6 -8，表明这两个物种的双C β基因系统具有很强的进化保守性。
Immune systems of vertebrates function via two types of effector cells, B and T cells, which are capable of antigen-specific recognition. The immunoglobulins, which serve as antigen receptors on B cells, have been well characterized with respect to gene structure1, unlike the T-cell receptors. Recently, cDNA clones thought to correspond to theβ-chain locus2–4of the human and mouse T-cell receptor have been described. The presumptiveβ-chain clones detect gene rearrangement specifically in T-cell DNA and show homology with immunoglobulin light chains. The similarity of the T-cellβ-chain gene system to the immunoglobulin genes has been further demonstrated by the recent observation of variable- and constant-region gene segments as well as joining segments and putative diversity segments5,6. We report here the characterization of cDNA and genomic clones encoding human T-cell receptorβ-chain genes. There are two constant-region genes (Cβ1 andCβ2), each capable of rearrangement and expression as RNA. The gene arrangement, analogous to that of mouseβ-chain genes6–8, shows strong evolutionary conservation of the dualCβgene system in these two species.