PATHOGENESIS OF SCRAPIE (STRAIN-263K) IN HAMSTERS INFECTED INTRACEREBRALLY, INTRAPERITONEALLY OR INTRAOCULARLY
PATHOGENESIS OF SCRAPIE (STRAIN-263K) IN HAMSTERS INFECTED INTRACEREBRALLY, INTRAPERITONEALLY OR INTRAOCULARLY
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DOI：
10.1099/0022-1317-67-2-255
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发表时间：
1986-02-01
影响因子：
3.8
通讯作者：
WALKER, CA
中科院分区：
文献类型：
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作者：
KIMBERLIN, RH;WALKER, CA
脑内（i.c.）感染仓鼠后，263 K羊瘙痒病菌株以几乎恒定的指数速率复制，直到脑内滴度平均为9.8 log 10 LD 50时出现临床疾病。单位/g。腹腔感染后，羊痒病复制首先在脾脏中检测到，然后在胸脊髓中，最后在腰脊髓和脑中检测到。这种模式提示中枢神经系统的侵袭是通过感染沿着某些内脏自主神经传播而发生的。仅在感染后3至4周（潜伏期16周）在胸髓中检测到免疫性，表明该瘙痒病模型的异常神经侵袭性。这一观察结果以及脾切除术未能延长潜伏期，增加了腹膜中神经组织直接感染并转移至胸髓的可能性，而瘙痒病病原体的先前复制很少。右眼眼内感染后，羊瘙痒病在右侧视神经和左侧上级丘中检测到复制（或积聚），然后在右侧上级丘中检测到复制（或积聚），最后在左侧视神经和髓质中检测到复制（或积聚）。这种模式表明羊瘙痒病感染可以沿着神经传播，可能是通过轴突内运输。病原体在脑内复制的持续时间（从复制开始到临床疾病）在腹腔内感染后最短（51 - 58天），在脑内感染后较长（81 - 88天），在眼内感染后最长（> 121天）。这些差异可能反映了神经通路的相对效率，通过这些神经通路，传染性从大脑中的不同进入部位传播到假定的“临床靶区”。
After intracerebral (i.c.) infection of hamsters, the 263K strain of scrapie replicated at a nearly constant exponential rate until clinical disease developed when titres in brain averaged 9.8 log10 LD50 i.c. units/g. After intraperitoneal infection, scrapie replication was first detected in spleen, then in thoracic spinal cord and finally in lumbar cord and brain. This pattern suggests that invasion of the central nervous system occurs by spread of infection along certain visceral autonomic nerves. Infectivity was detected in the thoracic cord only 3 to 4 weeks after infection (incubation period 16 weeks) indicating the exceptional neuroinvasiveness of this scrapie model. This observation and the failure of splenectomy to lengthen incubation period raises the possibility of direct infection of nerve tissue in the peritoneum and transport to the thoracic cord with minimal prior replication of scrapie agent extraneurally. After intraocular infection of the right eye, replication (or accumulation) of scrapie was detected in the right optic nerve and left superior colliculus, then in the right superior colliculus and finally in the left optic nerve and medulla. This pattern shows that scrapie infection can spread along nerves, possibly by intra-axonal transport. The duration of agent replication in brain (between detectable onset of replication and clinical disease) was shortest after intraperitoneal infection (51 to 58 days), longer after intracerebral infection (81 to 88 days) and longest after intraocular infection (> 121 days). These differences may reflect the relative efficiency of the neural pathways by which infectivity spreads from different sites of entry in the brain to the postulated ''clinical target areas''.