Identification of differentially expressed genes in senescence-accelerated mouse testes by suppression subtractive hybridization analysis
Identification of differentially expressed genes in senescence-accelerated mouse testes by suppression subtractive hybridization analysis
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DOI：
10.1007/s00335-006-0119-2
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发表时间：
2007-02-01
期刊：
影响因子：
2.5
通讯作者：
Higuchi, Keiichi
中科院分区：
文献类型：
--
作者：
Chiba, Takuya;Yao, Junjie;Higuchi, Keiichi
加速衰老小鼠（SAM）品系构成了一种加速衰老的模型，伴随着短寿命和各种年龄相关疾病的早期发展。为了鉴定加速衰老的SAMP 1和对照SAMR 1小鼠睾丸中的差异基因表达，我们进行了抑制性消减杂交。我们观察到，在SAMP 1小鼠中，与细胞增殖相关的三个基因（肌球蛋白调节轻链B、醛缩酶1A亚型和细胞色素c氧化酶亚基维克）的表达上调，与精子发生相关的四个基因下调。Asb-8是一种含有锚蛋白重复序列的蛋白质，在睾丸中大量表达，在SAMP 1中表达下调。其他三个下调的基因（生殖细胞特异性基因1，T-复合物多肽1b，和睾丸cAMP反应性调节因子的激活剂）已被报道调节晚期精子发生。这些基因表达谱可能解释了SAMP 1小鼠睾丸早期成熟和产生精子能力随年龄增长而迅速下降的结果。
Senescence-accelerated mouse (SAM) strains constitute a model of accelerated senescence coupled with a short lifespan and the early development of various age-related disorders. To identify differential gene expression in testes between senescence-accelerated SAMP1 and control SAMR1 mice, we performed suppression subtractive hybridization. We observed that the expression of three genes related to cell proliferation (myosin regulatory light chain B, aldolase 1A isoform, and cytochrome c oxidase subunit VIc) were upregulated and four genes implicated in spermatogenesis were downregulated in SAMP1 mice. Asb-8, a member of ankyrin repeat-containing proteins, was abundantly expressed in the testes and downregulated in SAMP1. The other three downregulated genes (germ cell-specific gene 1, T-complex polypeptide 1b, and activator of cAMP responsive clement modulator in testis) have been reported to regulate late-stage spermatogenesis. These gene expression profiles might explain the findings of early testicular maturation and rapid decline in the ability to produce spermatozoa with advancing age in SAMP1 mice.