Effect of Chronic Lead Exposure on Pro-Apoptotic Bax and Anti-Apoptotic Bcl-2 Protein Expression in Rat Hippocampus In Vivo
Effect of Chronic Lead Exposure on Pro-Apoptotic Bax and Anti-Apoptotic Bcl-2 Protein Expression in Rat Hippocampus In Vivo
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DOI：
10.1007/s10571-010-9504-1
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发表时间：
2010-07-01
影响因子：
4
通讯作者：
Jorjani, Masoumeh
中科院分区：
文献类型：
--
作者：
Sharifi, Ali M.;Mousavi, Seyed Hadi;Jorjani, Masoumeh
尽管环境中的铅减少，但长期铅暴露仍然具有公共健康危害，特别是在儿童中，对发育中的CNS具有破坏性影响。为了研究这种神经毒性的机制，年轻和成年大鼠被用来研究暴露于500 ppm浓度的铅是否会诱导海马细胞凋亡。2-4 12-14周龄的大鼠接受浓度为500 ppm的醋酸铅40天。对照动物接受去离子蒸馏水。染铅组血铅水平升高3-4倍。光镜和电镜下海马凋亡细胞增多。Bax和Bcl-2（分别为促凋亡和抗凋亡基因产物）的Western印迹分析表明，与对照组相比，Bax蛋白表达增加，而Bcl-2表达无显著变化，因此Bax/Bcl-2比值增加，证实了组织学研究。总之，这些数据表明，在体内海马慢性铅暴露的神经毒性可能部分是由于促进细胞凋亡。
Despite reduction in environmental lead, chronic lead exposure still possess a public health hazard, particularly in children, with devastating effects on developing CNS. To investigate the mechanism of this neurotoxicity, young and adult rats were used to study whether exposure to 500 ppm concentrations of lead could induce apoptosis in hippocampus. 2-4 and 12-14-week-old rats received lead acetate in concentration of 500 ppm for 40 days. Control animals received deionized distilled water. In lead-treated groups, the blood lead levels were increased by 3-4 folds. Light and electron microscopical study of hippocampus revealed increased apoptotic cells. Western blot analysis of Bax and Bcl-2 (pro- and anti-apoptotic gene products, respectively) indicated higher expression of Bax protein and no significant change in bcl-2 expression and accordingly increased the Bax/Bcl-2 ratio compared to control group, confirming the histological study. In conclusion, these data suggest that neurotoxicity of chronic lead exposure in hippocampus in vivo may partly be due to facilitation of apoptosis.