Introducing a Novel Analysis Technique for Osseointegrated Dental Implants Retrieved 29 Years Postsurgery
Introducing a Novel Analysis Technique for Osseointegrated Dental Implants Retrieved 29 Years Postsurgery
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DOI：
10.1111/j.1708-8208.2011.00413.x
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发表时间：
2013-08-01
影响因子：
3.6
通讯作者：
Johansson, Carina B.
中科院分区：
文献类型：
--
作者：
Sarve, Hamid;Friberg, Bertil;Johansson, Carina B.
目的：为了研究口腔种植患者29年后取出的种植体的骨整合情况，我们使用了新的三维分析方法和可视化技术，补充了传统的二维分析。材料和方法：样品处理包括不脱钙和在树脂中的包埋。采用常规二维组织形态计量学方法。此外，利用同步辐射显微计算机断层扫描(SRmCT)技术将定量扩展到三维，并介绍了两种相关的三维数据可视化方法。结果：三维结果包括两个种植体样本的三维量化和可视化，方法超出了最先进的方法。传统的二维组织形态计量学结果显示平均骨-种植体接触(BIC)约为50%。在大多数样本中，与外折叠镜面图像相比，胎面内的骨面积(BA)较小，这一点得到了三维定量的证实。四个选定区域的BIC在底部/谷区的百分比最高，在线峰区域的百分比最低。定性观察显示，所有样本中都有正在进行的骨改建区域。结论：新的技术包括动画和BIC和BA的外折叠，使得从SRmCT数据获得的三维材料能够同时可视化。然而，骨整合的定性和定量评估需要二维组织切片，因此，这两种方法被认为是同等重要的。
Purpose: To investigate osseointegration of oral implants, which were retrieved from a patient after 29 years in situ, we use novel three-dimensional analysis methods and visualization techniques that supplement conventional two-dimensional analysis.Materials and Methods: The sample processing involved nondecalcification and embedment in resin. Conventional two-dimensional histomorphometrical methods were conducted. Additionally, the quantification was extended to three-dimensional by using synchrotron radiation micro-computed tomography (SRmCT) technique and two relevant visualization methods for the three-dimensional data were introduced.Results: The three-dimensional results involved three-dimensional quantification and visualization of two implant samples with methods beyond state-of-the-art. Traditional two-dimensional histomorphometrical results revealed a mean bone-implant contact (BIC) of about 50%. In most samples, bone area (BA) was lower inside the treads compared with out-folded mirror images, which were confirmed by the three-dimensional quantification. The BIC along four selected regions showed highest percentages in the bottom/valley region and lowest in the thread-peak region. Qualitative observations revealed ongoing bone remodeling areas in all samples. The apical hole demonstrated high osseointegration.Conclusion: The novel techniques including an animation and an out-folding of BIC and BA enabled a simultaneous visualization of the three-dimensional material obtained from SRmCT data. However, the two-dimensional histological sections were needed for qualitative and quantitative evaluation of osseointegration and, thus, both methods are considered equally important.