CORNIFIED ENVELOPE OF TERMINALLY DIFFERENTIATED HUMAN EPIDERMAL KERATINOCYTES CONSISTS OF CROSS-LINKED PROTEIN
CORNIFIED ENVELOPE OF TERMINALLY DIFFERENTIATED HUMAN EPIDERMAL KERATINOCYTES CONSISTS OF CROSS-LINKED PROTEIN
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DOI：
10.1016/0092-8674(77)90059-9
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发表时间：
1977-01-01
期刊：
影响因子：
64.5
通讯作者：
GREEN, H
中科院分区：
文献类型：
--
作者：
RICE, RH;GREEN, H
人表皮愈伤组织角质层的一小部分蛋白质即使在含有十二烷基硫酸钠和还原剂的溶液中煮沸也不溶解。这种蛋白质存在于角化包膜中，角化包膜是位于质膜下方的结构。当通过彻底的蛋白水解消化溶解角质化的包膜并通过色谱法分析产物时，发现总赖氨酸残基的大约18％是交联二肽ε-(γ-谷氨酰)赖氨酸。标记的角质化包膜蛋白是由允许在培养物中终末分化的人表皮角质形成细胞合成的。由彻底蛋白水解后的ε-(γ-谷氨酰)赖氨酸中放射性赖氨酸的比例确定的交联程度与角质层中的交联程度相似。角化包膜的特性（不溶于去污剂和还原剂，以及蛋白水解消化后的溶解度）很容易通过由交联蛋白晶格组成的结构来解释。
A small proportion of the protein of stratum corneum of human epidermal callus is insoluble even when boiled in solutions containing sodium dodecylsulfate and a reducing agent. This protein is present in the cornified envelope, a structure located beneath the plasma membrane. When cornified envelopes were dissolved by exhaustive proteolytic digestion and the products analyzed by chromatography, approximately 18% of the total lysine residues were found at the cross-linking dipeptide .epsilon.-(.gamma.-glutamyl) lysine. Labeled cornified envelope protein was synthesized by human epidermal keratinocytes allowed to differentiate terminally in culture. The extent of cross-linking, determined from the proportion of radioactive lysine in .epsilon.-(.gamma.-glutamyl) lysine after exhaustive proteolysis, was similar to that in stratum corneum. The properties of the cornified envelopes (insolubility in detergent and reducing agents, and solubility following proteolytic digestion) are readily explained by a structure consisting of a cross-linked protein lattice.