Caries-preventive agents induce remineralization of dentin in a biofilm model
Caries-preventive agents induce remineralization of dentin in a biofilm model
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DOI：
10.1159/000084801
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发表时间：
2005-01-01
期刊：
影响因子：
4.2
通讯作者：
ten Cate, JM
中科院分区：
文献类型：
--
作者：
Deng, DM;van Loveren, C;ten Cate, JM
目的是研究生物膜下牙本质的矿化。这是在恒定深度膜发酵罐（CDFF）中进行的，该发酵罐经过改造，使得在一个实验中可以同时应用两种处理。45个变形链球菌生物膜生长在牙本质的凹槽中。将生长培养基（3.7g/l BHI、1.5mM钙和25 mM PIPES）与2%蔗糖脉冲交替施用4x 30分钟/天。仅以NaF形式或以与0.2%氯己定的混合物形式施加135 ppm的氟化物，持续2 x 5 min/天。接种后5天开始处理，持续15天。每组在不同时间点取出5个标本。检查生物膜的活力（通过平板接种）和酸含量（通过毛细管电泳）。牙本质标本进行了分析的矿物损失和病变深度（横向显微放射摄影）。氟化物处理对存活率没有影响，但减少了75%的乳酸产量。在第一天，混合物处理使活力降低80%，乳酸含量降低93%，随后使两个参数降低至检测限以下。治疗组之间观察到矿物质损失和病变深度的变化存在显著差异。氟化物组中观察到部分矿化但更深的病变，而混合物组中观察到几乎完全矿化。最后，CDFF S.变形菌生物膜模型可用作去矿化和去矿化生物膜模型，并且分离模式特别适合于测试防龋剂。版权所有（C）2005 S. Karger AG，巴塞尔。
The aim was to study remineralization in dentin underneath a biofilm. This was done in a constant depth film fermentor (CDFF) which was modified so that two treatments can be applied simultaneously in one experiment. Forty-five Streptococcus mutans biofilms were grown in grooves in dentin. Growth medium (3.7 g/I BHI, 1.5 mM calcium and 25 mM PIPES) was administered alternately with 2% sucrose pulsing 4 x 30 min/day. Fluoride at 135 ppm as NaF only or in a mixture with 0.2% chlorhexidine was applied for 2 x 5 min/day. The treatments started 5 days after inoculation and lasted 15 days. Five specimens per group were removed at various time points. The biofilms were checked for viability (by plating) and acid content (by capillary electrophoresis). The dentin specimens were analysed for mineral loss and lesion depth (by transversal microradiography). Fluoride treatment had no effect on the viability but reduced lactic acid production by 75%. The mixture treatment reduced the viability by 80% and the lactic acid content by 93% on the first day and later reduced the two parameters to below the detection limits. Significant differences in changes in mineral loss and lesion depth were observed between the treatment groups. Partial remineralization but deeper lesions were observed in the fluoride group, while nearly complete remineralization was seen in the mixture group. In conclusion, the CDFF S. mutans biofilm model can be used as a de- and remineralization biofilm model, and the split mode is particularly suitable for testing caries-preventive agents. Copyright (C) 2005 S. Karger AG, Basel.