Design principles of Cdr2 node patterns in fission yeast cells.
Design principles of Cdr2 node patterns in fission yeast cells.
复制标题
裂殖酵母细胞中Cdr2节点模式的设计原理。
DOI：
10.1101/2023.04.19.537536
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Moseley,JamesB
中科院分区：
文献类型：
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作者：
Opalko,Hannah;Geng,Shuhan;Hall,AaronR;Vavylonis,Dimitrios;Moseley,JamesB
模式形成网络在细胞生物学中具有不同的作用。杆状分裂酵母细胞使用模式形成来控制有丝分裂信号蛋白和细胞动力学环的定位。在间期，激酶Cdr 2形成膜结合的多蛋白复合物，称为节点，其位于细胞中间，部分原因是节点抑制剂Pom 1富集在细胞尖端。淋巴结定位对于及时的细胞周期进展和细胞动力学环的定位是重要的。在这里，我们结合实验和建模的方法来研究模式形成的Pom 1-Cdr 2系统。我们发现，Cdr 2节点附近的核积累，和Cdr 2经历核质穿梭时，皮质锚定减少。我们产生了基于粒子的模拟的基础上，尖端抑制，核定位和皮质锚定。我们通过在扰动每个定位机制（包括无核细胞和多核细胞）后研究Pom 1-Cdr 2定位模式来测试模型预测。实验表明，尖端抑制和皮质锚定单独是足够的组装和定位的节点的情况下的核，但核和Pom 1促进形成意想不到的节点模式在多核细胞。这些发现对节点对胞质分裂的空间控制和其他生物系统中的空间模式有影响。
Pattern-forming networks have diverse roles in cell biology. Rod-shaped fission yeast cells use pattern formation to control the localization of mitotic signaling proteins and the cytokinetic ring. During interphase, the kinase Cdr2 forms membrane-bound multiprotein complexes termed nodes, which are positioned in the cell middle due in part to the node inhibitor Pom1 enriched at cell tips. Node positioning is important for timely cell cycle progression and positioning of the cytokinetic ring. Here, we combined experimental and modeling approaches to investigate pattern formation by the Pom1-Cdr2 system. We found that Cdr2 nodes accumulate near the nucleus, and Cdr2 undergoes nucleocytoplasmic shuttling when cortical anchoring is reduced. We generated particle-based simulations based on tip inhibition, nuclear positioning, and cortical anchoring. We tested model predictions by investigating Pom1-Cdr2 localization patterns after perturbing each positioning mechanism, including in both anucleate and multinucleated cells. Experiments show that tip inhibition and cortical anchoring alone are sufficient for the assembly and positioning of nodes in the absence of the nucleus, but that the nucleus and Pom1 facilitate the formation of unexpected node patterns in multinucleated cells. These findings have implications for spatial control of cytokinesis by nodes and for spatial patterning in other biological systems.