The Paneth cell: a source of intestinal lysozyme.
The Paneth cell: a source of intestinal lysozyme.
复制标题
潘氏细胞：肠道溶菌酶的来源。
作者：
T. Peeters;G. Vantrappen
采用间接荧光抗体技术对小鼠小肠切片进行染色，制备了一种针对小鼠小肠粘膜刮擦物溶菌酶的抗血清。粘膜荧光仅限于Paneth细胞所在的Lieberkuhn隐窝底部。静脉注射4 mg匹罗卡品后，Paneth细胞中不再发现荧光，而是在隐窝腔中发现荧光。灌注研究证实了这些发现。静脉给药1 mg匹罗卡品后，基础溶菌酶产量从0-1 ~ 0-4马克/毫升提高到1-8 ~ 6-5马克/毫升的峰值。我们的研究结果表明，溶菌酶至少部分来源于Paneth细胞，并且可能存在于Paneth细胞颗粒中。它的分泌受到匹罗卡品的刺激。我们的模型可能对研究Paneth细胞的功能非常有用，Paneth细胞可能是肠道防御系统的一部分。
An antiserum prepared against lysozyme isolated from mucosal scrapings of mouse small intestine was used to stain sections of mouse small intestine with the indirect fluorescent antibody technique. Mucosal fluorescence was confined to the base of the crypts of Lieberkuhn, where Paneth cells are located. After the intravenous administration of 4 mg of pilocarpine fluorescence was no longer found in the Paneth cell but in the crypt lumen. Perfusion studies confirmed these findings. The basal lysozyme output of 0-1 to 0-4 mug/ml was raised to peak rates of 1-8 to 6-5 mug/ml after the intravenous administration of 1 mg of pilocarpine. Our results demonstrate that the lysozyme of the succus entericus is, at least in part, derived from the Paneth cell, and is probably present in the Paneth cell granules. Its secretion is stimulated by pilocarpine. Our model could be very useful for studying the function of the Paneth cell, which probably forms part of an intestinal defence system.