Towards a specific characterisation of components on a cell surface - combined TERS-investigations of lipids and human cells
Towards a specific characterisation of components on a cell surface - combined TERS-investigations of lipids and human cells
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DOI：
10.1002/jrs.2433
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发表时间：
2009-10-01
影响因子：
2.5
通讯作者：
Popp, J.
中科院分区：
文献类型：
--
作者：
Boehme, R.;Richter, M.;Popp, J.
采用尖端增强拉曼光谱(TERS)技术研究了负载型脂质结构和人体细胞(人真皮来源角质形成细胞，HaCaT)，以确定细胞表面的特定成分。由于脂类是细胞膜的主要成分，因此研究了纯脂的光谱性质与复杂生物样品的相关性。在动态结构变化和纳米尺度效应的诱导下，发现脂质TERS光谱的一个特殊光谱特征发生了变化，在细胞上测量的层次光谱中出现了类似的光谱偏差。单靠对细胞表面的修饰并不能导致这种行为。与软脂团相反，细胞是固定的，因此阻碍了内在结构的变化。因此，细胞层光谱变化的主要原因是纳米尺度效应，这是由与传统拉曼光谱不同的光谱特性决定的。目前的结果表明，TERS能够提供对细胞表面组成的详细和快速的洞察，甚至允许检测单个组分。版权所有(C)2009 John Wiley&Sons，Ltd.
Supported lipid structures and human cells (human dermal derived keratinocyte, HaCaT) were investigated using tip-enhanced Raman spectroscopy (TERS) to use the high spatial resolution capabilities of TIERS, which is assumed to be less than 10 nm, to determine specific components on the cell surface. As lipids are a main component of cellular membranes, the correlation of spectral properties of pure lipids with respect to the complex biological sample was investigated. Induced by dynamic structural changes as well as nanoscale effects, a particular spectral feature of the lipid TERS spectra is found to vary, and a similar spectral deviation appears among the TIERS spectra measured on the cell. Modifications of the cell surface alone cannot cause such behaviour. In contrast to soft lipid agglomerates, the cells were fixed and therefore hampered for intrinsic structural changes. Hence, the main contribution for the cell TIERS spectra variation results from nanoscale effects, determined by different spectral characteristics compared to conventional Raman spectroscopy. The present results demonstrate the capability of TERS to provide a detailed and fast insight into the composition of the cell surface, even allowing the detection of single components. Copyright (C) 2009 John Wiley & Sons, Ltd.