Effects of Phycosphere Bacteria on Their Algal Host Are Host Species-Specific and Not Phylogenetically Conserved.
Effects of Phycosphere Bacteria on Their Algal Host Are Host Species-Specific and Not Phylogenetically Conserved.
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DOI：
10.3390/microorganisms11010062
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发表时间：
2022-12-25
期刊：
影响因子：
4.5
通讯作者：
Denef, Vincent J.
中科院分区：
文献类型：
--
作者：
Baker, Dylan;Lauer, James;Ortega, Anna;Jackrel, Sara L.;Denef, Vincent J.
浮游植物是地球生命的基础。它们的生产力受到藻类细胞周围藻圈中微生物群落的影响。将我们关于藻-菌相互作用如何影响藻类生长的知识扩展到更多的宿主和细菌可以帮助阐明藻-宿主相互作用的一般原理。在这里，我们从藻圈群落中分离出368株细菌菌株，在从池塘水中招募藻圈后，经过一个月的实验室培养，并研究了它们对五种绿色藻类生长的影响。我们分离出丰富和罕见的藻圈成员，占18.4%的源社区。对寄主生长的影响以正效应和中性效应为主，而非负效应。各效应类型的比例以及隔离日期是否重要因寄主种类而异。细菌对藻类承载力的影响大于对藻类生长速率的影响，这表明营养盐的矿化和有毒副产物的代谢可能是一个主导机制。跨宿主藻类健身测定表明，我们的分离物的主机特定的生长效果。我们没有观察到细菌分离株对宿主生长的影响的系统发育保守性。甚至具有相同ASV的分离物对宿主生长也有不同的影响。我们的研究结果强调了藻圈中高度特异性的宿主-细菌相互作用，并提出了哪些机制介导这些相互作用的问题。
Phytoplankton is fundamental to life on Earth. Their productivity is influenced by the microbial communities residing in the phycosphere surrounding algal cells. Expanding our knowledge on how algal-bacterial interactions affect algal growth to more hosts and bacteria can help elucidate general principles of algal-host interactions. Here, we isolated 368 bacterial strains from phycosphere communities, right after phycosphere recruitment from pond water and after a month of lab cultivation and examined their impacts on growth of five green algal species. We isolated both abundant and rare phycosphere members, representing 18.4% of the source communities. Positive and neutral effects predominated over negative effects on host growth. The proportion of each effect type and whether the day of isolation mattered varied by host species. Bacteria affected algal carrying capacity more than growth rate, suggesting that nutrient remineralization and toxic byproduct metabolism may be a dominant mechanism. Across-host algal fitness assays indicated host-specific growth effects of our isolates. We observed no phylogenetic conservation of the effect on host growth among bacterial isolates. Even isolates with the same ASV had divergent effects on host growth. Our results emphasize highly specific host-bacterial interactions in the phycosphere and raise questions as to which mechanisms mediate these interactions.
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影响因子：
64.8
作者：
Amin, S. A.;Hmelo, L. R.;Armbrust, E. V.
通讯作者：
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影响因子：
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通讯作者：
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作者：
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