EFFICACY OF INACTIVATED INFLUENZA-A VIRUS (H3N2) VACCINES GROWN IN MAMMALIAN-CELLS OR EMBRYONATED EGGS
EFFICACY OF INACTIVATED INFLUENZA-A VIRUS (H3N2) VACCINES GROWN IN MAMMALIAN-CELLS OR EMBRYONATED EGGS
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DOI：
10.1093/infdis/160.2.191
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发表时间：
1989-08-01
影响因子：
6.4
通讯作者：
WEBSTER, RG
中科院分区：
文献类型：
--
作者：
KATZ, JM;WEBSTER, RG
从单个感染个体分离的流感病毒（H3 N2）宿主细胞变体在雪貂中用作福尔马林灭活的纯化全病毒疫苗时比较了它们的保护效力。在鸡胚卵中生长的A/Mem/12/85病毒（卵生长的）与在哺乳动物Madin-Darby犬肾细胞中生长的A/Mem/12/85病毒（MDCK生长的）的不同之处在于血凝素分子中的单个氨基酸取代，免疫雪貂血清显示出可区分性。用完整的灭活MDCK-或卵生长病毒肌内免疫雪貂，随后用在任一宿主细胞类型中生长的感染性病毒进行攻击。MDCK-生长的病毒疫苗诱导更高的平均血清血凝抑制（HAI）和中和抗体滴度比鸡蛋生长的病毒疫苗和诱导上级保护雪貂对随后的挑战与感染性病毒生长在任一宿主细胞类型。这些结果表明，与在哺乳动物细胞中生长的病毒在抗原性和结构上相似的人流感病毒作为疫苗可能比在鸡蛋中选择的一些变体更有效。
Influenza virus (H3N2) host cell variants isolated from a single infected individual were compared for their protective efficacies when used as a formalin-inactivated purified whole virus vaccines in ferrets. A/Mem/12/85 virus grown in embryonic chicken eggs (egg-grown), which differs from A/Mem/12/85 grown in mammalian Madin-Darby canine kidney cells (MDCK-grown) by a single amino acid substitution in the hemagglutinin molecule, was shown to be distinguishable by immune ferret serum. Ferrets were immunized intramuscularly with intact inactivated MDCK- or egg-grown virus and were subsequently challenged with infectious virus grown in either host cell type. MDCK-grown-virus vaccine induced higher mean serum hemagglutination-inhibiting (HAI) and neutralizing antibody titers than did egg-grown-virus vaccine and induced superior protection of ferrets against subsequent challenge with infectious virus grown in either host cell type. These results suggest that human influenza viruses that are antigenically and structurally similar to viruses grown in mammalian cells may be more efficacious as vaccines than some variants selected in eggs.