Colocalization of delta sleep inducing peptide and luteinizing hormone releasing hormone in neurosecretory vesicles in rat median eminence.
Colocalization of delta sleep inducing peptide and luteinizing hormone releasing hormone in neurosecretory vesicles in rat median eminence.
复制标题
大鼠正中隆起神经分泌囊泡中 Delta 睡眠诱导肽和黄体生成素释放激素的共定位。
DOI：
10.1159/000125705
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发表时间：
1991
影响因子：
4.1
通讯作者：
J. Z. Kiss
中科院分区：
文献类型：
--
作者：
P. Vallet;Yves Charnay;Constantin Boura;J. Z. Kiss
应用光镜和电镜免疫细胞化学方法，观察了大鼠正中隆起内类似δ睡眠诱导肽（DSIP）和促黄体生成素释放激素（LH-RH）的免疫反应共定位。在光镜水平，DSIP和LH-RH免疫染色纤维，静脉曲张表现出类似的分布模式，整个正中隆起。免疫反应阳性的轴突主要分布在外层的外侧部。使用洗脱-再染色技术，LH-RH和DSIP免疫反应性的共存观察到在大多数标记的轴突。为了确定DSIP和LH-RH的细胞内定位，我们使用了种特异性抗体和与不同大小的金颗粒缀合的二抗的双重免疫细胞化学标记。发现这两种肽共定位于单个轴突中。免疫反应性终末经常表现为直接膜附着与伸展细胞过程，但很少接触门静脉毛细血管。在伸长细胞和室管膜或神经胶质细胞中未观察到染色。此外，我们可以证明LH-RH和DSIP（或密切相关的分子形式）不仅包含在相同的轴突中，而且还包含在相同的约100 nm的致密核心囊泡中，这表明这些肽的共同分泌。
The colocalization of immunoreactivities similar to delta sleep inducing peptide (DSIP) and luteinizing hormone releasing hormone (LH-RH) was investigated by light and electron microscopic immunocytochemistry in the rat median eminence. At the light microscopic level, DSIP and LH-RH immunostained fibers, and varicosities exhibited a similar distribution pattern throughout the median eminence. Immunoreactive axons were mainly found in the lateral part of the external layer. Using an elution-restaining technique, the coexistence of LH-RH and DSIP immunoreactivities was observed in most labelled axons. To determine the intracellular localization of DSIP and LH-RH, we used double immunocytochemical labelling with species-specific antibodies and secondary antibodies conjugated to different sizes of gold particles. The two peptides were found colocalized in single axons. Immunoreactive terminals frequently showed direct membrane apposition with tanycyte processes but rare contacts with portal capillaries. No staining was observed in tanycytes and ependymal or glial elements. Moreover, we could demonstrate that LH-RH and DSIP (or a closely related molecular form) are contained not only in the same axons, but also in the same approximately 100-nm dense-core vesicles, suggesting cosecretion of these peptides.