The anatomy and physiology of spray cut chrysanthemum pedicels, and expression of a caffeic acid 3-O-methyltransferase homologue
The anatomy and physiology of spray cut chrysanthemum pedicels, and expression of a caffeic acid 3-O-methyltransferase homologue
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喷雾切菊花梗的解剖学和生理学以及咖啡酸3-O-甲基转移酶同源物的表达
DOI：
10.1016/j.postharvbio.2011.01.004
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发表时间：
2011-06-01
影响因子：
7
通讯作者：
Chen, Sumei
中科院分区：
文献类型：
--
作者：
Lv, Guosheng;Tang, Dejuan;Chen, Sumei
喷切菊花（Chrysanthemum grandiflorum（Ramat.）Kitamura）是一种受欢迎的切花品种。花梗硬度是决定菊花采后品质的重要因素，以2个菊花品种为材料，对花梗在花芽发育各阶段的形态、解剖和生理进行了比较研究。具有较好瓶插寿命的品种（“H5”）的花梗在整个开花阶段显示出较高的维管成分比例，并且在阶段II和III期间具有较高的木质素和相对含水量。从菊花中分离到一个编码咖啡酸3-O-甲基转移酶（CgCOMT）的cDNA。它包含一个1065 bp的开放阅读框，预测编码355个残基的多肽。CgCOMT产物的推导肽序列与从其他植物物种分离的COMT蛋白非常相似，并且共享S-腺苷甲硫氨酸底物结合基序LVDVGGGXG。通过qRT-PCR的表达分析表明，CgCOMT在整个植物中表达，但在茎中特别显著。随着花梗的发育，该基因的转录量增加，在瓶插寿命较短的品种‘H5’中的转录量高于‘QX 101’。这一观察结果与它们的花梗硬度的差异是一致的。(C)2011 Elsevier B. V.保留所有权利。
The spray cut chrysanthemum (Chrysanthemum grandiflorum (Ramat.) Kitamura) is a popular cut flower species. As pedicel rigidity is a key component of its postharvest quality, the morphology, anatomy and physiology of the pedicel at various stages of flower bud development were compared in two contrasting chrysanthemum cultivars. The pedicel of the cultivar with the better vase life ('H5') showed a higher proportion of vascular elements throughout all floral stages, and higher lignin and relative water contents during stages II and III. A cDNA encoding caffeic acid 3-O-methyltransferase (CgCOMT) was isolated from chrysanthemum. It comprises a 1065 bp opening read frame, predicted to encode a 355 residue polypeptide. The deduced peptide sequence of the CgCOMT product is very similar to that of COMT proteins isolated from other plant species, and shares the S-adenosylmethionine substrate binding motif LVDVGGGXG. Expression analysis by qRT-PCR showed that CgCOMT was expressed throughout the plant, but was particularly prominently in the stem. Its transcription increased with the development of the pedicel, and was higher in 'H5' than in 'QX101', the cultivar with the lesser vase life. This observation was consistent with the differences in rigidity of their pedicels. (C) 2011 Elsevier B.V. All rights reserved.