Rapid Detection of Polymyxin Resistance in Enterobacteriaceae.
Rapid Detection of Polymyxin Resistance in Enterobacteriaceae.
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DOI：
10.3201/eid2206.151840
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发表时间：
2016-06
影响因子：
11.8
通讯作者：
Poirel L
中科院分区：
文献类型：
--
作者：
Nordmann P;Jayol A;Poirel L
该测试价格低廉，易于执行，灵敏、特异，可在2小时内完成。为了鉴定肠杆菌科细菌对多粘菌素的耐药性，我们开发了一种快速检测方法，可以在一定浓度的粘菌素或多粘菌素B存在的情况下检测与细菌生长相关的葡萄糖代谢。酸性代谢物的形成是通过pH指示剂(红色苯酚)的颜色变化(橙色到黄色)来证明的。为了评估测试，我们使用了135个表达粘菌素耐药机制(固有的、染色体编码的和质粒介导的MCR-1)的菌株和65个粘菌素敏感菌株的细菌菌落。与标准肉汤微量稀释法比较，灵敏度为99.3%，特异度为95.4%。这种新的测试价格低廉，易于执行，灵敏、特异，可以在两个小时内完成。它可能对面临碳青霉烯酶生产者地方性传播的国家有用，对这些国家来说，多粘菌素是最后的药物。
The test is inexpensive, easy to perform, sensitive, specific, and can be completed in <2 hours. For identification of polymyxin resistance in Enterobacteriaceae, we developed a rapid test that detects glucose metabolization associated with bacterial growth in the presence of a defined concentration of colistin or polymyxin B. Formation of acid metabolites is evidenced by a color change (orange to yellow) of a pH indicator (red phenol). To evaluate the test, we used bacterial colonies of 135 isolates expressing various mechanisms of colistin resistance (intrinsic, chromosomally encoded, and plasmid-mediated MCR-1) and 65 colistin-susceptible isolates. Sensitivity and specificity were 99.3% and 95.4%, respectively, compared with the standard broth microdilution method. This new test is inexpensive, easy to perform, sensitive, specific, and can be completed in <2 hours. It could be useful in countries facing endemic spread of carbapenemase producers and for which polymyxins are last-resort drugs.