Evaluation of a multiplex PCR to identify and serotype Actinobacillus pleuropneumoniae serovars 1, 5, 7, 12 and 15
Evaluation of a multiplex PCR to identify and serotype Actinobacillus pleuropneumoniae serovars 1, 5, 7, 12 and 15
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DOI：
10.1111/lam.12287
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发表时间：
2014-10-01
影响因子：
2.4
通讯作者：
Blackall, P. J.
中科院分区：
文献类型：
--
作者：
Turni, C.;Singh, R.;Blackall, P. J.
研究的意义和影响：开发并验证了可以识别胸膜肺炎放线菌血清型 1、5、7、12 和 15 的多重 PCR。这种新型诊断工具将使一线实验室能够提供关键信息（血清型），以指导猪胸膜肺炎（一种严重的猪经济疾病）的有针对性的预防和控制计划。以前的技术，即传统的血清分型，通常由专门的参考实验室提供，限制了应对这种关键疾病的能力。
Significance and Impact of the Study: A multiplex PCR that can recognize serovars 1, 5, 7, 12 and 15 of A. pleuropneumoniae was developed and validated. This novel diagnostic tool will enable frontline laboratories to provide key information (the serovar) to guide targeted prevention and control programmes for porcine pleuropneumonia, a serious economic disease of pigs. The previous technology, traditional serotyping, is typically provided by specialized reference laboratories, limiting the capacity to respond to this key disease.