XSIP1 is essential for early neural gene expression and neural differentiation by suppression of BMP signaling
XSIP1 is essential for early neural gene expression and neural differentiation by suppression of BMP signaling
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DOI：
10.1016/j.ydbio.2004.08.010
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发表时间：
2004-11-01
影响因子：
2.7
通讯作者：
Asashima, M
中科院分区：
文献类型：
--
作者：
Nitta, KR;Tanegashima, K;Asashima, M
神经分化是通过抑制BMP信号传导而诱导的。从Spemann组织体分泌的BMP抑制剂如Chordin有助于神经诱导的第一步。Xenopus Smad-interacting protein-1 gene （XSIP1）在爪蟾的神经外胚层从原肠胚早期到神经胚期均有表达。XSIP1能够抑制BMP信号传导，过表达XSIP1诱导神经分化。为了阐明XSIP1在神经分化中的功能，我们对XSIP1进行了功能缺失研究。敲低XSIP1抑制SoxD表达和神经分化。这些结果表明XSIP1在神经诱导中是必不可少的。此外，功能缺失实验表明，SoxD对XSIP1转录和神经分化至关重要。然而，抑制XSIP1翻译可阻止SoxD诱导的神经分化；因此，SoxD不足以介导神经分化。抑制BMP信号也需要XSIP1的表达。总之，这些结果表明XSIP1和SoxD相互依赖地维持神经分化。(C) 2004爱思唯尔公司版权所有。
Neural differentiation is induced by inhibition of BMP signaling. Secreted inhibitors of BMP such as Chordin from the Spemann organizer contribute to the initial step of neural induction. Xenopus Smad-interacting protein-1 gene (XSIP1) is expressed in neurroectoderm from the early gastrula stage through to the neurula stage. XSIP1 is able to inhibit BMP signaling and overexpression of XSIP1 induces neural differentiation. To clarify the function of XSIP1 in neural differentiation, we performed a loss-of-function study of XSIP1. Knockdown of XSIP1 inhibited SoxD expression and neural differentiation. These results indicate that XSIP1 is essential for neural induction. Furthermore, loss-of-function experiments showed that SoxD is essential for XSIP1 transcription and for neural differentiation. However, inhibition of XSIP1 translation prevented neural differentiation induced by SoxD; thus, SoxD was not sufficient to mediate neural differentiation. Expression of XSIP1 was also required for inhibition of BMP signaling. Together, these results suggest that XSIP1 and SoxD interdependently function to maintain neural differentiation. (C) 2004 Elsevier Inc. All rights reserved.