Synovial‐type (group II) phospholipase A2 human seminal plasma
Synovial‐type (group II) phospholipase A2 human seminal plasma
复制标题
滑液型（II 组）磷脂酶 A2 人精浆
作者：
T. Nevalainen;K. Meri;M. Niemi
概括。对生育力、生育力低下和输精管切除男性的精浆以及前列腺分泌物和组织中的磷脂酶 A2 (PLA2) 进行了分析。在滑膜型 PLA2 抗血清和精浆中存在的酶之间观察到免疫交叉反应性。通过时间分辨荧光免疫测定法测量，滑膜型 PLA2 的浓度与 PLA2 的催化活性之间存在高度显着的相关性。结果表明，人精浆中的PLA2含量非常高（约为血浆中的1000倍），并且该酶属于滑液型II型磷脂酶A2。结果还表明该酶是由前列腺分泌的。
Summary. Phospholipase A2 (PLA2) was analysed in seminal plasma of fertile, subfertile, and vasectomized men as well as in prostatic secretion and tissue. Immunological cross‐reactivity was observed between synovial‐type PLA2 antiserum and the enzyme present in seminal plasma. There was a highly significant correlation between the concentration of the synovial‐type PLA2, as measured by a time‐resolved fluoroimmunoassay and the catalytic activity of the PLA2. The results show that the PLA2 content in human seminal plasma is very high (approximately 1000 times of that present in blood plasma) and that the enzyme belongs to the synovial‐type group II phospholipase A2. The results also indicate that the enzyme is secreted by the prostate.