Insulinoma-associated protein 1 immunostaining on cytology specimens: an institutional experience
Insulinoma-associated protein 1 immunostaining on cytology specimens: an institutional experience
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DOI：
10.1016/j.humpath.2018.10.035
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发表时间：
2019-03-01
期刊：
影响因子：
3.3
通讯作者：
Maleki, Zahra
中科院分区：
文献类型：
--
作者：
Rodriguez, Erika F.;Fite, J. Judd;Maleki, Zahra
神经内分泌肿瘤（NETs）是具有显著神经内分泌分化的上皮肿瘤。细胞学检查和免疫组化（IHC）标记的使用是重要的诊断工具，这些肿瘤的评价。在这里，我们报告我们的经验与INSM 1细胞学样本的应用。我们在我们的病理学系统中检索了2017年至2018年进行的INSM 1 IHC细胞学标本。记录患者的人口统计学资料，并审查细胞学材料，包括进行的所有神经内分泌IHC标记物。共识别出134份（67例男性，67例女性）INSM 1 IHC标本。样本包括91例（68.2%）神经内分泌肿瘤或具有神经内分泌特征的肿瘤（TNEF），33例（24.3%）非神经内分泌病变（非NET）和10例（7.5%）非肿瘤诊断。INSM 1在90例（99%）NET/TNEF中呈阳性，在32例（97%）非NET中呈阴性。42例（95.5%）NET/TNEFs中CD 56阳性，9例（69.2%）非NET/TNEFs中CD 56阴性。INSM 1的敏感性为99%，特异性为97%; CD 56的敏感性为95.5%，特异性为69.2%。嗜铬粒蛋白的敏感性最低（82.5%），突触素的特异性最低（66.7%）。INSM 1的阳性和阴性预测值均高于CD 56（分别为99%和97%）。INSM 1是一种敏感和特异的标记物，用于检测细胞学样本中的NETs，而不依赖于原发位点。(C)2018爱思唯尔公司All rights reserved.
Neuroendocrine tumors (NETs) are epithelial neoplasms with prominent neuroendocrine differentiation. Cytologic examination and utilization of immunohistochemical (IHC) markers are important diagnostic tools for the evaluation of these tumors. Herein we report our experience with the application of INSM1 in cytology samples. We searched our pathology system for cytologic specimens with INSM1 IHC performed from 2017 to 2018. Patients' demographics were recorded, and cytology materials were reviewed including all neuroendocrine IHC markers performed. A total of 134 (67 male, 67 female) specimens with INSM1 IHC were identified. Specimens included 91 (68.2%) NETs or tumors with neuroendocrine features (TNEFs), 33 (24.3%) nonneuroendocrine lesions (non-NET), and 10 (7.5%) nonneoplastic diagnoses. INSM1 was positive in 90 (99%) of the NET/TNEFs and negative in 32 (97%) non-NETs. CD56 was positive in 42 (95.5%) of the NET/TNEFs and negative on 9 (69.2%) of the non-NETs. The sensitivity of INSM1 was 99% and specificity was 97%, whereas the sensitivity of CD56 was 95.5% and specificity was 69.2%. Chromogranin had the lowest sensitivity (82.5%), and synaptophysin had the lowest specificity (66.7%). Both positive and negative predictive values of INSM1 were higher than CD56 (99% versus 91.3% and 97% versus 81.8%, respectively). INSM1 is a sensitive and specific marker for detection of NETs in cytology samples independent of primary site. (C) 2018 Elsevier Inc. All rights reserved.