Optimization of culture medium for cordycepin production using Cordyceps militaris mutant obtained by ion beam irradiation
Optimization of culture medium for cordycepin production using Cordyceps militaris mutant obtained by ion beam irradiation
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DOI：
10.1016/j.procbio.2009.08.008
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发表时间：
2010-01-01
影响因子：
4.4
通讯作者：
Sakakibara, Mikio
中科院分区：
文献类型：
--
作者：
Das, Shonkor Kumar;Masuda, Mina;Sakakibara, Mikio
研究了不同培养基组分对离子束辐照北虫夏草突变株生产虫草素的影响。采用中心复合设计对拟突变株G81-3进行响应面分析，得到以葡萄糖为碳源的最佳浓度为86.2g/L，以酵母膏为氮源的最佳浓度为93.8g/L，得到虫草素的最适浓度为6.84g/L。到目前为止，这是虫草素生产的最高价值。突变株产虫草素的最适葡萄糖和酵母膏浓度明显高于对照(野生株)，虫草素产量是对照菌株的2.79倍。因此，这一新的突变株将成为未来更高水平的虫草素工业化生产的有前途的菌株。(C)2009爱思唯尔有限公司。保留所有权利。
The effect of medium components on cordycepin production by Cordyceps militaris mutant obtained by ion beam irradiation was investigated. According to the response surface analysis using a central composite design for the prospective mutant G81-3, the predicted optimal concentrations of glucose as the carbon source and the yeast extract as the nitrogen source were 86.2 g/l and 93.8 g/l, respectively, and 6.84 g/l cordycepin was obtained. To date, this is the highest Value for cordycepin production. The optimal concentrations of glucose and yeast extract for cordycepin production of the mutant was much higher than that of control (wild strain) and the cordycepin production was 2.79 times higher. Therefore, this new mutant will be a promising strain for future higher cordycepin production at industrial levels. (C) 2009 Elsevier Ltd. All rights reserved.