NUCLEOTIDE-SEQUENCE OF THE REPRESSOR GENE OF THE TN10 TETRACYCLINE RESISTANCE DETERMINANT
NUCLEOTIDE-SEQUENCE OF THE REPRESSOR GENE OF THE TN10 TETRACYCLINE RESISTANCE DETERMINANT
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DOI：
10.1093/nar/12.12.4849
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发表时间：
1984-01-01
影响因子：
14.9
通讯作者：
BERTRAND, KP
中科院分区：
文献类型：
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作者：
POSTLE, K;NGUYEN, TT;BERTRAND, KP
Tn 10 tetR基因编码调节Tn 10四环素抗性决定簇转录的阻遏物。我们测定了tetR基因的DNA序列，并在tetR基因3′端有一个905碱基对的区域。TetR基因位于701个碱基对的HincII限制性片段上。该区域两端的缺失消除了E中野生型tetR蛋白的合成。大肠杆菌亚胺细胞，并通过β-半乳糖苷酶合成intetA-lacZ操纵子融合菌株的阻遏来测量消除R活性。总的来说，DNA序列和遗传数据表明tetR编码一个207个氨基酸的蛋白质，计算分子量为23，328。该值与基于SDS-聚丙烯酰胺凝胶电泳迁移率的估计值23，000 - 25，000非常一致。Tn 10蛋白与RP 1/Tn 1721 tetR蛋白推导的氨基酸序列有47%的同源性。此外，Tnl 0 tetR阻遏物的NH 2-末端区域与其它DNA结合蛋白的DNA识别区域之间存在显著的氨基酸序列同源性。
The Tn10 tetRgene encodes the repressor that regulates transcription of the Tnl0tetracycline resistance determinant. We have determined the DNA sequence of thetetRgene and a 905 base pair region immediately 3′ totetR. ThetetRgene is located on a 701 base pairHincII restriction fragment. Deletions at either end of this region eliminate synthesis of the wild-typetetRprotein inE. coliminicells, and eliminatetetRactivity as measured by repression of β-galactosidase synthesis intetA-lacZoperon fusion strains. Taken together, the DNA sequence and the genetic data indicate thattetRencodes a 207 amino acid protein with a calculated molecular weight of 23,328. This value is in good agreement with estimates of 23,000–25,000 based on electrophoretic mobility in SDS-polyaerylamide gels. There is 47% amino acid sequence homology between the deduced sequences of the Tn10and RPl/Tnl721tetRproteins. There is, in addition, significant amino acid sequence homology between an NH2-terminal region of the Tnl0tetRrepressor and the DNA recognition regions of other DNA-binding proteins.