Vitamin C increases the formation of prostacyclin by aortic rings from various species and neutralizes the inhibitory effect of 15‐hydroperoxy‐arachidonic acid
Vitamin C increases the formation of prostacyclin by aortic rings from various species and neutralizes the inhibitory effect of 15‐hydroperoxy‐arachidonic acid
复制标题
维生素 C 促进不同物种的主动脉环形成前列环素，并中和 15-氢过氧-花生四烯酸的抑制作用
DOI：
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发表时间：
1983
影响因子：
7.3
通讯作者：
A. Herman
中科院分区：
文献类型：
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作者：
J. Beetens;A. Herman
1大鼠、家兔和豚鼠的主动脉环在30分钟内产生不同数量的6‐oxo‐PGF1α(6‐oxo‐PGF1α)，这是前列腺素的稳定分解产物，即每mg湿重2760±195、160±10和87±17 pg 6‐oxo‐PGF1α。2维生素C可以促进这三种动物的主动脉组织产生6‐oxo‐PGF1α，与它们的基底释放无关。只有在孵育前和孵育液中都存在维生素C时，这种增加才显着。15 -氢过氧花生四烯酸抑制6 -氧- PGF1α的生成（IC50:6 μm），这种抑制作用被维生素c完全中和。4 6 -氧- PGF1α生成的增加不是由于底物花生四烯酸释放的增加。这表明维生素C通过保护环加氧酶和PGI合酶来促进6 - oxo - PGF1α的形成。
1 Aortic rings from rats, rabbits and guinea‐pigs produce different amounts of 6‐oxo***‐prostaglandin F1α (6‐oxo‐PGF1α), the stable breakdown product of prostacyclin, i.e. 2760 ± 195, 160 ± 10 and 87 ± 17 pg 6‐oxo‐PGF1α per mg wet weight in 30 min. 2 Vitamin C enhances the production of 6‐oxo‐PGF1α by the aortic tissue of these three species, independent of their basal release. This increase was only significant if vitamin C was present in the preincubation as well as in the incubation fluid. 3 15‐Hydroperoxy‐arachidonic acid inhibits the production of 6‐oxo‐PGF1α (IC50:6 μm) and this inhibitory effect was completely neutralized by vitamin C. 4 The increased production of 6‐oxo‐PGF1α is not due to an increased release of the substrate arachidonic acid. It is suggested that vitamin C enhances the formation of 6‐oxo‐PGF1α by protecting the cyclo‐oxygenase and PGI‐synthase.