Patch clamp recording from bipolar cells in the wholemount mouse retina.
Patch clamp recording from bipolar cells in the wholemount mouse retina.
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DOI：
10.1016/j.xpro.2022.101482
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发表时间：
2022-09-16
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双极细胞是视网膜中的二级神经元，较难被用来研究它们的突触反应。在这里，我们提出了一种从Ai32突变小鼠的全视网膜双极细胞进行膜片钳记录的方案。我们详细介绍了双极细胞的全细胞膜片钳记录，以检查它们对光遗传刺激的光诱发反应，然后记录细胞的成像终端来确定双极细胞的类型。我们描述了光刺激信息来激活通道视紫红质-2(ChR2)。有关本议定书的使用和执行的完整细节，请参阅。完整小鼠视网膜双极细胞膜片钳记录的详细协议活体视网膜组织中双极细胞亚型的识别详细光刺激信息通道视紫红质(ChR2)激活出版商备注：实施任何实验协议都需要遵守当地实验室安全和伦理的机构指南。双极细胞是视网膜中的二级神经元，较难被用来研究它们的突触反应。在这里，我们提出了一种从Ai32突变小鼠的全视网膜双极细胞进行膜片钳记录的方案。我们详细介绍了双极细胞的全细胞膜片钳记录，以检查它们对光遗传刺激的光诱发反应，然后记录细胞的成像终端来确定双极细胞的类型。我们描述了光刺激信息来激活通道视紫红质-2(ChR2)。
Bipolar cells are the second-order neurons in the retina that are less accessible for investigating their synaptic responses. Here, we present a protocol to conduct patch clamp recordings from bipolar cells in the wholemount retina from Ai32 mutant mice. We detail whole-cell patch-clamp recording from bipolar cells to examine their light-evoked responses to optogenetic stimulation, followed by imaging terminals of recorded cells to determine bipolar cell type. We describe light stimulus information to activate channelrhodopsin-2 (ChR2). For complete details on the use and execution of this protocol, please refer to. Detailed protocol for bipolar cell patch clamp recordings in wholemount mouse retina Bipolar cell subtype identification in live retinal tissue Detailed light stimulus information for channelrhodopsin (ChR2) activation Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Bipolar cells are the second-order neurons in the retina that are less accessible for investigating their synaptic responses. Here, we present a protocol to conduct patch clamp recordings from bipolar cells in the wholemount retina from Ai32 mutant mice. We detail whole-cell patch-clamp recording from bipolar cells to examine their light-evoked responses to optogenetic stimulation, followed by imaging terminals of recorded cells to determine bipolar cell type. We describe light stimulus information to activate channelrhodopsin-2 (ChR2).