Expression of Nogo protein by growing axons in the developing nervous system
Expression of Nogo protein by growing axons in the developing nervous system
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DOI：
10.1016/s0169-328x(02)00172-9
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发表时间：
2002-08-15
期刊：
影响因子：
--
通讯作者：
Hirata, T
中科院分区：
文献类型：
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作者：
Tozaki, H;Kawasaki, T;Hirata, T
我们制备了一种单克隆抗体NG1，它能强烈结合小鼠发育中的神经系统中的生长轴突。该抗体强烈标记培养神经元的生长锥。尽管这些免疫染色模式提示生长的轴突与该抗体识别的抗原有关，但抗原被鉴定为Nogo蛋白，一种从髓鞘中分离出来的轴突排斥因子。基于这一意想不到的发现，我们讨论了Nogo在发育中的神经系统中的可能功能。(C) 2002 Elsevier Science B.V.版权所有
We produced monoclonal antibody NG1 that strongly binds growing axons in the developing nervous system of mice. This antibody intensely labeled the growth cone of cultured neurons. Although these immunostaining patterns suggested the association of growing axons with the antigen recognized by this antibody, the antigen was identified as Nogo protein, an axonal repulsive factor isolated from the myelin. On the basis of this unexpected finding, we discuss the possible functions of Nogo in the developing nervous system. (C) 2002 Elsevier Science B.V. All rights reserved.