Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophila
Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophila
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DOI：
10.1038/nbt1212
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发表时间：
2006-06-01
影响因子：
46.9
通讯作者：
Boccard, Frédéric
中科院分区：
文献类型：
--
作者：
Vodovar, Nicolas;Vallenet, David;Boccard, Frédéric
嗜虫假单胞菌是一种昆虫病原细菌，在摄入后，杀死黑腹果蝇以及来自不同目的昆虫。测定了5.9-Mb基因组的完整序列，并与四种假单胞菌的测序基因组进行了比较。嗜虫假单胞菌具有与恶臭假单胞菌KT 2440密切相关的大部分分解代谢基因，揭示了其代谢多样性和土壤生活方式。揭示了可能与其昆虫病原特性有关的几个特征。对于动物病原体而言，意想不到的是，嗜虫假单胞菌缺乏III型分泌系统和相关毒素，而是依赖于许多潜在的毒力因子，如杀虫毒素、蛋白酶、推定的溶血素、氰化氢和新的次级代谢产物来感染和杀死昆虫。全基因组随机诱变揭示了GacS/GacA双组分系统的主要作用，该系统调节大多数已确定的潜在毒力因子。
Pseudomonas entomophila is an entomopathogenic bacterium that, upon ingestion, kills Drosophila melanogaster as well as insects from different orders. The complete sequence of the 5.9-Mb genome was determined and compared to the sequenced genomes of four Pseudomonas species. P. entomophila possesses most of the catabolic genes of the closely related strain P. putida KT2440, revealing its metabolically versatile properties and its soil lifestyle. Several features that probably contribute to its entomopathogenic properties were disclosed. Unexpectedly for an animal pathogen, P. entomophila is devoid of a type III secretion system and associated toxins but rather relies on a number of potential virulence factors such as insecticidal toxins, proteases, putative hemolysins, hydrogen cyanide and novel secondary metabolites to infect and kill insects. Genome-wide random mutagenesis revealed the major role of the two-component system GacS/GacA that regulates most of the potential virulence factors identified.