Late measures of brain injury after neonatal hypoxia-ischemia in mice
Late measures of brain injury after neonatal hypoxia-ischemia in mice
复制标题
DOI：
10.1161/01.str.0000137768.25203.df
复制
发表时间：
2004-09-01
期刊：
影响因子：
8.3
通讯作者：
Pinsky, DJ
中科院分区：
文献类型：
--
作者：
Ten, VS;Wu, EX;Pinsky, DJ
背景和目的-这项工作进行，以确定在何种程度上的长期神经功能的结果新生儿缺氧缺血性（HI）脑损伤的小鼠相关的解剖程度的脑损伤评估磁共振成像（MRI）和组织病理学。在7至9周后HI神经功能的结果进行了评估，水迷宫，旋转棒，和开放领域的测试性能，然后由脑MRI和组织病理学evaluation.Results -在10周后HI，MRI显示同侧脑萎缩单独或与脑穿通囊肿形成和对侧脑室扩大。成年HI感染小鼠，特别是那些发展成脑穿通囊肿，表现出显着的神经功能缺损与年龄匹配的幼稚小鼠相比。HI影响的小鼠同侧脑萎缩，但没有表现出任何或中等水平的神经功能缺损。神经行为评估的小鼠进行HI侮辱揭示了脑损伤和功能neurohandicap.Conclusions的程度之间的强相关性-这是第一项研究表明，长期的神经功能结果在小鼠新生儿HI后密切相关的解剖模式/脑损伤的程度，定义由MRI和组织病理学。
Background and Purpose - This work was undertaken to determine to what degree long-term neurofunctional outcome of neonatal hypoxic - ischemic ( HI) brain injury in mice correlates with anatomical extent of cerebral damage assessed by magnetic resonance imaging (MRI) and histopathology.Methods - On postnatal day 7, mice were subjected to HI. At 7 to 9 weeks after HI neurofunctional outcome was assessed by water-maze, rota-rod, and open-field test performance, followed by cerebral MRI and histopathology evaluation.Results - At 10 weeks after HI, MRI revealed ipsilateral brain atrophy alone or with porencephalic cyst formation and contralateral ventriculomegaly. Adult HI-affected mice, especially those that developed a porencephalic cyst, demonstrated significant neurofunctional deficit compared with age-matched naive mice. HI-affected mice with ipsilateral cerebral atrophy but without porencephaly demonstrated no or an intermediate level of neurofunctional deficit. Neurobehavioral assessment of mice subjected to HI insult revealed a strong correlation between degree of brain injury and functional neurohandicap.Conclusions - This is the first study to demonstrate that long-term neurofunctional outcome in mice after a neonatal HI correlates tightly with anatomical pattern/extent of cerebral damage, defined by MRI and histopathology.