Prevalence of hepatitis B and hepatitis D coinfection in asymptomatic blood donors in Iran
Prevalence of hepatitis B and hepatitis D coinfection in asymptomatic blood donors in Iran
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DOI：
10.1111/apm.12137
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发表时间：
2014-03-01
期刊：
影响因子：
2.8
通讯作者：
Ataee, Zahrah
中科院分区：
文献类型：
--
作者：
Attaran, Mona Seyed;Sharifi, Zohreh;Ataee, Zahrah
丁型肝炎病毒（HDV）是一种卫星病毒，需要乙型肝炎病毒（HBV）表面抗原进行扩增和转变。 HDV 在 HBsAg 携带者中表现为慢性乙型肝炎患者的急性合并感染和二重感染。这种合并感染会导致慢性肝炎、肝硬化和肝癌。本研究的目的是检测伊朗献血者中乙型肝炎病毒和丁型肝炎病毒双重感染和双重感染的患病率。对伊朗国家输血组织储存的阳性样本库中 854 名无症状献血者的血清进行了分析，这些献血者的乙型肝炎表面抗原呈阳性。使用 ELISA 检测献血者体内是否存在抗 HDV 抗体，然后进行常规 PCR、半巢式 PCR 和实时 PCR 以确定共感染和/或重复感染。限制性片段长度多态性用于 HDV 基因分型。所有 854 个样本均为 HBsAg 和抗 HBc 阳性，而其中只有 18 个（2%）抗 HDV 呈阳性。在 854 个样本中，154 个（18%）HBV-DNA 呈阳性。通过半巢式PCR和实时PCR，在总样本的0.6%中检测到HDV-RNA，两种PCR方法产生相似的结果。此外，抗 HDV 阳性样本中分别有 16.6% 和 83.4% 表现出 HBV 共感染和重复感染。在阳性样本中确定了 HDV 基因型 I。
Hepatitis delta virus (HDV) is a satellite virus that needs hepatitis B virus (HBV) surface antigen for amplification and transition. HDV appears in HBsAg carriers as acute coinfection and superinfection in patients with chronic hepatitis B. This coinfection leads to chronic hepatitis, cirrhosis, and liver carcinoma. The aim of this study was to detect the prevalence of coinfection and superinfection of HBVs and HDVs in blood donor individuals in Iran. Sera from 854 asymptomatic blood donors from the Bank of positive samples storage at the National Blood Transfusion Organization of Iran that were positive for hepatitis B surface antigen were analysed. The presence of antibody against HDV in blood donors was detected using ELISA followed by conventional PCR, seminested PCR and real-time PCR to determine coinfection and/or superinfection. Restriction fragment length polymorphism was used for HDV genotyping. All 854 samples were HBsAg and anti-HBc positive whereas only 18 (2%) of them were positive for anti-HDV. Of the 854 samples, 154 (18%) were HBV-DNA positive. HDV-RNA was detected in 0.6% of the total samples by seminested PCR and real-time PCR and the two PCR methods produced similar results. Moreover, 16.6% and 83.4% of anti-HDV-positive samples exhibited coinfection and superinfection with HBV, respectively. Genotype I of HDV was determined in positive samples.