ISOLATION OF HIGHLY ACIDIC AND VANADIUM-CONTAINING BLOOD-CELLS FROM AMONG SEVERAL TYPES OF BLOOD-CELL FROM ASCIDIIDAE SPECIES BY DENSITY-GRADIENT CENTRIFUGATION
ISOLATION OF HIGHLY ACIDIC AND VANADIUM-CONTAINING BLOOD-CELLS FROM AMONG SEVERAL TYPES OF BLOOD-CELL FROM ASCIDIIDAE SPECIES BY DENSITY-GRADIENT CENTRIFUGATION
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DOI：
10.1002/jez.1402570304
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发表时间：
1991-03-01
影响因子：
--
通讯作者：
HIRATA, J
中科院分区：
文献类型：
--
作者：
MICHIBATA, H;IWATA, Y;HIRATA, J
报道的海鞘血细胞的pH值变化很大。 很可能几种不同类型的血细胞中的一种或两种必须在它们的空泡内具有高度酸性的溶液，其中钒离子将以还原状态存在。 因此，报告的pH值变化的原因之一可能是在没有分离总细胞群的情况下进行pH测量。 本实验采用密度梯度离心分离全细胞，微电极法测定厌氧条件下的pH值，原子吸收光谱法测定钒的含量，结果表明，三种海鞘（Ascidiagemmata，A. ahodori和A. sydneiensis samea的pH值较低，分别为2.4、2.7和4.2。 通过ESR（电子自旋共振）光谱法获得的结果证实了用微电极获得的pH值。
The reported pH values of ascidian blood cells vary considerably. It is likely that one or two of several different types of blood cell must have a highly acidic solution within their vacuoles, in which vanadium ions would be present in a reduced state. Thus, one of the reasons for the variation in reported pH values may be that measurements of pH were made without fractionation of the total population of cells. In the present experiments, the combination of fractionation of whole cells by density-gradient centrifugation, use of a microelectrode that allows measurements of pH under anaerobic conditions, and atomic absorption spectrometry for determinations of vanadium revealed that the contents of signet ring cells, recently identified as vanadocytes, of three ascidian species, Ascidia gemmata, A. ahodori, and A. sydneiensis samea, had low pH values of 2.4, 2.7, and 4.2, respectively. Results obtained by ESR (electron spin resonance) spectrometry confirmed the values of pH obtained with the microelectrode.